A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction

被引：36

作者：

Huang, Ngan F.

Sievers, Richard E.

Park, Jennifer S.

Fang, Qizhi

Li, Song

Lee, Randall J. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Joint Bioengn Grad Grp, Berkeley, CA 94720 USA

[3] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA

[4] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nprot.2006.188

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a robust rat model of myocardial infarction (MI). Here we describe the step-by-step protocol for creating an ischemia-reperfusion rat model of MI. We also describe how to deliver therapeutic injections of mesenchymal stem cells (MSCs) together with fibrin, to show an application of this model. In addition, to confirm the presence of fibrin and cells in the infarct, visualization of MSCs and fibrin by histological techniques are also described. The ischemia-reperfusion MI model can be modified and generalized for use with various injectable polymers, cell types, drugs, DNA and combinations thereof. The model can be created in 7 days or less, depending on the timing of therapeutic intervention.

引用

页码：1596 / 1609

页数：14

共 31 条

[1]

Azizi Michel, 2004, Rev Prat, V54, P1167

[2] Transplantation of autologous endothelial cells induces angiogenesis [J].