Measurement of 3-hydroxyisovaleric acid in urine of biotin-deficient infants and mice by HPLC

被引：10
|
作者：
Watanabe, T [1 ]
Oguchi, KI
Ebara, S
Fukui, T
机构：
[1] Univ Hyogo, Himeji Inst Technol, Sch Human Sci & Environm, Himeji, Hyogo 6700092, Japan
[2] Byotai Seiri Lab, Tokyo 1730025, Japan
来源：
JOURNAL OF NUTRITION | 2005年 / 135卷 / 03期
关键词：
3-hydroxyisovaleric acid; HPLC; biotin deficiency; methylcrotonyl CoA carboxylase; urine;
D O I：
10.1093/jn/135.3.615
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
We developed an assay for measuring urinary 3-hydroxyisovaleric acid (3-HIA) using HPLC after derivatization with 2-nitrophenylhydrazine hydrochloride (2-NPH . HCl). The derivatized 3-HIA was extracted into n-hexane and separated isocratically on a C8 reversed-phase column for fatty acids (YMC-Pack FA). We used this method to measure 3-HIA in urine extracts from mice fed a biotin-deficient diet for > 4 wk and in an infant who was fed a special Japanese formula and was suspected of being biotin deficient. Urinary 3-HIA could be assayed within the range of 0.42-8.5 mmol/L with high accuracy by this method, as an indicator of biotin deficiency. Therefore, the HPLC method for 3-HIA described here may be a useful tool clinically as well as in the research laboratory.
引用
收藏
页码：615 / 618
页数：4
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