Replicative competence of the T131I, K141E, and G145R surface variants of hepatitis B virus

被引：11

作者：

Jammeh, Saffie ^{[1
]}

Thomas, Howard C. ^{[1
]}

Karayiannis, Peter ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci & Technol, Dept Med, Div Med, Hepatol Sect, London W2 1NY, England

来源：

JOURNAL OF INFECTIOUS DISEASES | 2007年 / 196卷 / 07期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1086/521198

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Variants of hepatitis B surface antigen have been described in different clinical settings, but their replicative capacity in vitro has remained unexplored. Point mutations leading to sT131I, sK141E, and sG145R amino-acid substitutions were engineered by site-directed mutagenesis into an infectious plasmid clone of the virus. The mutated constructs were transfected into Huh7 cells, and their replication capacity was documented by LightCycler ( Roche Diagnostics) measurements of virion-associated hepatitis B virus (HBV) DNA, intracellular relaxed circular double-stranded DNA, and pregenomic RNA. The sT131I and sG145R variants replicated with efficiency equal to that of the wild type, whereas the sK141E variant was replication impaired.

引用

页码：1010 / 1013

页数：4

共 15 条

[1] VACCINE-INDUCED ESCAPE MUTANT OF HEPATITIS-B VIRUS [J].