Accessibility of tyrosine YZ. to exogenous reductants and Mn2+ in various Photosystem II preparations

被引:8
|
作者
Chroni, S [1 ]
Ghanotakis, DF [1 ]
机构
[1] Univ Crete, Dept Chem, Heraklion 71409, Crete, Greece
来源
关键词
Photosystem II; tyrosine Y-Z; exogenous reductant; manganese complex;
D O I
10.1016/S0005-2728(01)00159-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reduction of tyrosine Y-Z(.) by benzidine and exogenous Mn2+ was studied by kinetic EPR experiments in various Photosystem II (PSII) preparations. Using lanthanide treated PSII membranes it was demonstrated that neither the extrinsic polypeptides (17, 23 and 33 kDa) nor the Mn complex block the accessibility of Y-Z(.) to exogenous reductants, such as benzidine. In addition, it was shown that in the presence of the native Mn complex exogenous Mn2+ does not reduce Y-Z(.). (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:432 / 437
页数:6
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