Surface-enhanced Raman scattering biosensor-based sandwich-type for facile and sensitive detection of Staphylococcus aureus

被引:26
|
作者
Wei, Wenya [1 ]
Haruna, Suleiman A. [1 ]
Zhao, Yumeng [1 ]
Li, Huanhuan [1 ]
Chen, Quansheng [1 ,2 ]
机构
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[2] Jimei Univ, Coll Food & Biol Engn, Xiamen 361021, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
SERS; Staphylococcus aureus; Polydimethylsiloxane; Vancomycin; Core-shell nanoparticles; SELECTIVE DETECTION; FLUORESCENCE;
D O I
10.1016/j.snb.2022.131929
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Foodborne infections caused by Staphylococcus aureus (S. aureus) represent a serious health risk to the general public. In this study, we proposed a surface-enhanced Raman spectroscopy (SERS) biosensor based on a signal amplification sandwich-type system for detecting and quantifying S. aureus. Herein, the SERS response of SiO2- coated Au@Ag was studied using Raman signals from Au@Ag@SiO2 nanoparticles and van grafted PDMS for the target acquisition. It has been shown that SiO2 coated Au@Ag exhibited a robust and strong response to SERS. Therefore, the combination of vancomycin (Van) and polydimethylsiloxane (PDMS) allowed a more efficient capture of S. aureus, resulting in improved repeatability through simplified separation procedures. Consequently, as a result of the presence of S. aureus targets, sandwich-type conjugate structures between the capture and signal units, as well as synergistic Raman amplification, were observed. Based on optimized conditions, a good linear correlation (y = 1087.86x 540.75, R-2 = 0.9958) in a wide dynamic range of 38-3.8 x 107 cfu.mL(-1) was achieved with a limit of detection of 2 cfu.mL(-1). The accuracy of the sensors was evaluated using a standard addition technique, and the results were satisfactory. Finally, the overall biosensor demonstrated potential application for monitoring S. aureus with good recoveries.
引用
收藏
页数:10
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