One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

被引：73

作者：

Sun, Yuanyuan ^{[1
]}

Tian, Hui ^{[1
]}

Liu, Chenghui ^{[1
]}

Sun, Yueying ^{[1
]}

Li, Zhengping ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem,Minist Educ, Xian 710062, Shaanxi, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 80期

基金：

中国国家自然科学基金;

关键词：

ULTRASENSITIVE DETECTION; SINGLE-CELL; LIGATION; DNA; QUANTIFICATION; PROBES;

D O I：

10.1039/c7cc06140d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.

引用

收藏

页码：11040 / 11043

页数：4

相关论文

共 27 条

[1] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[2] Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification [J].

Cheng, Yongqiang ;

Zhang, Xian ;

Li, Zhengping ;

Jiao, Xiaoxia ;

Wang, Yucong ;

Zhang, Yali .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (18) :3268-3272

[3] Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification [J].

Duan, Ruixue ;

Zuo, Xiaolei ;

Wang, Shutao ;

Quan, Xiyun ;

Chen, Dongliang ;

Chen, Zhifei ;

Jiang, Lei ;

Fan, Chunhai ;

Xia, Fan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (12) :4604-4607

[4] Oncomirs - microRNAs with a role in cancer [J].

Esquela-Kerscher, A ;

Slack, FJ .

NATURE REVIEWS CANCER, 2006, 6 (04) :259-269

[5] Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) [J].

Hsieh, Kuangwen ;

Mage, Peter L. ;

Csordas, Andrew T. ;

Eisenstein, Michael ;

Soh, H. Tom .

CHEMICAL COMMUNICATIONS, 2014, 50 (28) :3747-3749

[6] Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification [J].

Jia, Hongxia ;

Li, Zhengping ;

Liu, Chenghui ;

Cheng, Yongqiang .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (32) :5498-5501

[7] A microRNA detection system based on padlock probes and rolling circle amplification [J].

Jonstrup, Soren Peter ;

Koch, Jorn ;

Kjems, Jorgen .

RNA, 2006, 12 (09) :1747-1752

[8] Discriminating single-base difference miRNA expressions using microarray Probe Design Guru (ProDeG) [J].

Lee, Inhan ;

Ajay, Subramanian S. ;

Chen, Haiming ;

Maruyama, Atsushi ;

Wang, Nulang ;

McInnis, Melvin G. ;

Athey, Brian D. .

NUCLEIC ACIDS RESEARCH, 2008, 36 (05)

[9] One-step ultrasensitive detection of microRNAs with loop-mediated isothermal amplification (LAMP) [J].

Li, Cuiping ;

Li, Zhengping ;

Jia, Hongxia ;

Yan, Jingli .

CHEMICAL COMMUNICATIONS, 2011, 47 (09) :2595-2597

[10] Multiplex Loop-Mediated Isothermal Amplification Detection by Sequence-Based Barcodes Coupled with Nicking Endonuclease-Mediated Pyrosequencing [J].

Liang, Chao ;

Chu, Yanan ;

Cheng, Sijia ;

Wu, Haiping ;

Kajiyama, Tomoharu ;

Kambara, Hideki ;

Zhou, Guohua .

ANALYTICAL CHEMISTRY, 2012, 84 (08) :3758-3763