Neuraminidase inhibitors susceptibility profiles of highly pathogenic influenza A (H5N1) viruses isolated from avian species in India (2006-2015)

被引:6
|
作者
Sood, Richa [1 ]
Kumar, Naveen [1 ]
Bhatia, Sandeep [1 ]
Chanu, Khangembam Victoria [1 ,3 ]
Gupta, Chhedi Lal [2 ]
Pateriya, Atul Kumar [1 ]
Mishra, Anamika [1 ]
Khandia, Rekha [1 ,4 ]
Mawale, Namrata [1 ]
Singh, Vijendra Pal [1 ]
机构
[1] ICAR Natl Inst High Secur Anim Dis, Bhopal 462022, Madhya Pradesh, India
[2] UCL, Dept Comp Sci, Gower St, London WC1E 6BT, England
[3] ICAR Directorate Cold water Fisheries, Bhimtal 263136, Nainital, India
[4] Barkatullah Univ, Dept Biochem & Genet, Bhopal 462026, India
关键词
HPAI A(H5N1) viruses; Neuraminidase inhibitors; Oseltamivir; Zanamivir; India; ANTIVIRAL SUSCEPTIBILITY; REDUCED SENSITIVITY; OSELTAMIVIR; RESISTANCE; EMERGENCE; ZANAMIVIR; POULTRY; SUBTYPE;
D O I
10.1016/j.antiviral.2018.08.007
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
We tested 65 highly pathogenic avian influenza (HPAI) A(H5N1) viruses, isolated from avian species in India between 2006 and 2015, for susceptibility to the FDA approved neuraminidase (NA) inhibitors (NAIs), oseltamivir and zanamivir using a phenotypic fluorescence-based assay. The overall incidence of resistant variants among HPAI A(H5N1) viruses was 7.69% (5/65). The NA inhibition assay identified 3 viruses resistant to oseltamivir (N294S substitution, N2 numbering) and 2 cross-resistant to oseltamivir and zanamivir (E119A or 1117V + E119A substitutions), all of which belonged to hemagglutinin (HA) Glade 2.2 (5/17) and predominantly circulated in Indian poultry during 2006-2010. In comparison to E119A substitution alone, viruses with 1117V + E119A double substitutions showed greater reduction in susceptibility to both oseltamivir and zanamivir. The NAI resistance-associated NA markers, identified in this study, were as a result of naturally occurring mutations. Of note, 48 viruses of HA Glade 2.3.2.1 that circulated in Indian poultry during 2011-2015 were susceptible to both oseltamivir and zanamivir. It is essential to monitor NAI susceptibility among human and avian HPAI A(H5N1) viruses that would provide baseline data to develop strategies for pandemic preparedness and therapeutic interventions.
引用
收藏
页码:143 / 146
页数:4
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