Effect of soluble soybean protein hydrolysate-calcium complexes on calcium uptake by Caco-2 cells

被引：35

作者：

Lv, Y. ^{[1
]}

Bao, X. -L. ^{[1
]}

Yang, B. -C. ^{[1
]}

Ren, C. -G. ^{[1
]}

Guo, S. -T. ^{[1
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

JOURNAL OF FOOD SCIENCE | 2008年 / 73卷 / 07期

关键词：

Caco-2; cells; calcium uptake; molecular weight; soybean protein hydrolysates;

D O I：

10.1111/j.1750-3841.2008.00873.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Soybean protein hydrolysates (SPHs) bind with calcium, forming soluble SPH-calcium complexes via the carboxyl groups of glutamic and aspartic acid residues. However, their effect on calcium uptake is still unclear. In this study, Caco-2 cells were used to estimate the effect of SPH-calcium complexes with different molecular weights on calcium uptake in vitro. The changes in intracellular calcium ion concentration were measured by Fura-2 loading and expressed in fluorescence intensity. SPH-calcium complexes could promote calcium uptake. Improved fluorescence intensity was significantly different in SPH-calcium complexes (10 to 30 kDa), SPH-calcium complexes (3 to 10 kDa), and SPH-calcium complexes (I to 3 kDa). The maximum levels of relative fluorescence intensity (18.3) occurred with SPH-calcium complexes (10 to 30 kDa). The effect of SPH-calcium complexes (10 to 30 kDa) on Ca2+ increase was determined to be concentration dependent in the range of 0.5 to 4 mg/mL. Our results indicate that soybean protein itself might be responsible for promoting calcium absorption.

引用

页码：H168 / H173

页数：6

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