Effect of soluble soybean protein hydrolysate-calcium complexes on calcium uptake by Caco-2 cells

被引：35
|
作者：
Lv, Y. [1 ]
Bao, X. -L. [1 ]
Yang, B. -C. [1 ]
Ren, C. -G. [1 ]
Guo, S. -T. [1 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
来源：
JOURNAL OF FOOD SCIENCE | 2008年 / 73卷 / 07期
关键词：
Caco-2; cells; calcium uptake; molecular weight; soybean protein hydrolysates;
D O I：
10.1111/j.1750-3841.2008.00873.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Soybean protein hydrolysates (SPHs) bind with calcium, forming soluble SPH-calcium complexes via the carboxyl groups of glutamic and aspartic acid residues. However, their effect on calcium uptake is still unclear. In this study, Caco-2 cells were used to estimate the effect of SPH-calcium complexes with different molecular weights on calcium uptake in vitro. The changes in intracellular calcium ion concentration were measured by Fura-2 loading and expressed in fluorescence intensity. SPH-calcium complexes could promote calcium uptake. Improved fluorescence intensity was significantly different in SPH-calcium complexes (10 to 30 kDa), SPH-calcium complexes (3 to 10 kDa), and SPH-calcium complexes (I to 3 kDa). The maximum levels of relative fluorescence intensity (18.3) occurred with SPH-calcium complexes (10 to 30 kDa). The effect of SPH-calcium complexes (10 to 30 kDa) on Ca2+ increase was determined to be concentration dependent in the range of 0.5 to 4 mg/mL. Our results indicate that soybean protein itself might be responsible for promoting calcium absorption.
引用
收藏
页码：H168 / H173
页数：6
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