Silanimonas algicola sp nov., isolated from laboratory culture of a bloom-forming cyanobacterium, Microcystis

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated M23(T), was isolated from a laboratory culture of a bloom-forming cyanobacterium, Microcystis, which was isolated from a eutrophic lake in Korea. The strain grew optimally without NaCl and at 25-30 degrees C on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the genus Silanimonas, with the highest similarity to Silanimonas lenta DSM 16282(T) (98.5 %). DNA-DNA relatedness between strain M23(T) and the closely related species in the genus Silanimonas was < 30 %. Strain M23(T) contained iso-C-15 : 0, summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) and iso-C-16 : 0 as major fatty acids and ubiquinone-8 (Q-8) as the major quinone. Strain M23(T) contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmethylethanolamine as major polar lipids. The DNA G+C content of strain M23(T) was 69.6 mol%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain M23(T) represents a novel species in the genus Silanimonas, for which the name Silanimonas algicola sp. nov. is proposed. The type strain is M23(T) (= KCTC 52219(T) = JCM 31889(T))