Microbacterium ureisolvens sp. nov., isolated from a Yellow River sample

A Gram-stain positive, aerobic, non-motile and short-rod-shaped strain, CFH S00084(T), was isolated from a sediment sample of the Yellow River in Henan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CFH S00084(T) clustered within members of Microbacterium and was most closely related to the type strains Microbacterium yannicii JCM 18959(T) and Microbacterium arthrosphaerae DSM 22421(T)(98.97 % and 98.36 % similarity, respectively). The strain grew optimally at 25-37 degrees C, at pH 7.0 and in 0-3 % (w/v) NaCl. The major whole-cell sugars were rhamnose and glucose. The cell-wall peptidoglycan mainly contained glycine, alanine and ornithine. The menaquinones of strain CFH S00084(T) were MK-13, MK-12 and MK-11. The major fatty acids detected were anteiso-C-15:0, anteiso-C-17:0 and iso-C-16:0. The genome of strain CFH S00084(T) was 4.03 Mbp with a G+C content of 70.5 mol%. The average nucleotide identity (ANI) and digital DNA- DNA hybridization (dDDH) values between CFH S00084(T) and the other species of the genus Microbacterium were found to be low (ANIm <85 %, ANIb <75 % and dDDH <24 %). The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain CFH S00084(T) and its closely related type strains. On the basis of the differential physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH S00084(T) is considered to represent a novel species for which the name Microbacterium ureisolvens sp. nov. is proposed. The type strain is CFH S00084(T) (=KCTC 39802(T )=DSM 103157(T)).