Localized surface plasmon resonance based gold nanobiosensor: Determination of thyroid stimulating hormone

被引：19

作者：

Salahvarzi, Afsaneh ^{[1
]}

Mahani, Mohamad ^{[1
]}

Torkzadeh-Mahani, Masoud ^{[2
]}

Alizadeh, Reza ^{[3
]}

机构：

[1] Grad Univ Adv Technol, Fac Sci & Modern Technol, Dept Chem & Nanochem, Kerman, Iran

[2] Grad Univ Adv Technol, Inst Sci High Technol & Environm Sci, Dept Biotechnol, Kerman, Iran

[3] Univ Qom, Dept Chem, Fac Sci, Qom, Iran

来源：

ANALYTICAL BIOCHEMISTRY | 2017年 / 516卷

关键词：

Gold nanoparticles; Localized surface plasmon resonance; Nanobiosensor; Sonochemical synthesis; Nanoprobes; Thyroid stimulating hormone; DIELECTRIC ENVIRONMENT; METAL NANOPARTICLES; ENZYME-IMMUNOASSAY; SHAPE; THYROTROPIN; ASSAYS; SERUM; TSH;

D O I：

10.1016/j.ab.2016.10.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4-12.5 mIU L-1 and the calibration sensitivity of 1.71 L mILI(-1) were obtained. The human control serum sample was analyzed for TSH by constructed nanobiosensor and the acceptable results were obtained. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：1 / 5

页数：5

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