Clonal Dissemination of Salmonella enterica Serovar Infantis in Germany

被引：64
作者：
Hauser, Elisabeth [1 ,2 ]
Tietze, Erhard [3 ]
Helmuth, Reiner [1 ]
Junker, Ernst [1 ]
Prager, Rita [3 ]
Schroeter, Andreas [1 ]
Rabsch, Wolfgang [3 ]
Fruth, Angelika [3 ]
Toboldt, Anne [1 ]
Malorny, Burkhard [1 ]
机构：
[1] Natl Reference Lab Salmonella, Fed Inst Risk Assessment, Biol Safety Dept, D-12277 Berlin, Germany
[2] Univ Hohenheim, Dept Food Microbiol, Inst Food Sci & Biotechnol, D-7000 Stuttgart, Germany
[3] Natl Reference Ctr Salmonellae & Other Enter, Div Bacterial Infect, Wernigerode Branch, Robert Koch Inst, Wernigerode, Germany
来源：
FOODBORNE PATHOGENS AND DISEASE | 2012年 / 9卷 / 04期
关键词：
FIELD GEL-ELECTROPHORESIS; ANTIMICROBIAL RESISTANCE; SEROTYPE INFANTIS; BROILER-CHICKENS; HUMANS; EMERGENCE; POULTRY; MEAT;
D O I：
10.1089/fpd.2011.1038
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Salmonella enterica serovar Infantis (Salmonella Infantis) is consistently isolated from broiler chickens, pigs, and humans worldwide. This study investigated 93 epidemiologically unrelated Salmonella Infantis strains isolated in Germany between 2005 and 2008 in respect to their transmission along the food chain. Various phenotypic and genotypic methods were applied, and the pathogenicity and resistance gene repertoire was determined. Phenotypically, 66% of the strains were susceptible to all 17 antimicrobials tested, while the others were almost all multidrug-resistant (two or more antimicrobial resistances), with different resistance profiles and preferentially isolated from broiler chickens. A number of phage types (PTs) were shared by strains from pigs, broiler chickens, and humans (predominated by PT 29). One, PT 1, was only detected in strains from pigs/pork and humans. Pulsed-field gel electrophoresis (PFGE) subdivided strains in seven different clusters, named A-G, consisting of 35 various XbaI profiles with coefficient of similarity values of 0.73-0.97. The majority of XbaI profiles were assigned to clusters A and C, and two predominant XbaI profiles were common in strains isolated from all sources investigated. Multi-locus sequence typing (MLST) analysis of selected strains representing the seven PFGE clusters revealed that they all belonged to ST32. The pathogenicity gene repertoire of 37 representative Salmonella Infantis strains analyzed by microarray was also identical. The resistance gene repertoire correlated perfectly with the phenotypic antimicrobial resistance profiles, and multidrug-resistant strains were associated with class 1 integrons. Overall, this study showed that two major closely related genotypes of Salmonella Infantis can transmit in Germany to humans through contaminated broiler meat or pork, and consequently presents a hazard for human health.
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页码：352 / 360
页数：9
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