Induction of germline transcription in the human TCRγ locus by STAT5

被引:20
|
作者
Lee, HC [1 ]
Ye, SK [1 ]
Honjo, T [1 ]
Ikuta, K [1 ]
机构
[1] Kyoto Univ, Grad Sch Med, Dept Med Chem, Sakyo Ku, Kyoto 6068501, Japan
来源
JOURNAL OF IMMUNOLOGY | 2001年 / 167卷 / 01期
关键词
D O I
10.4049/jimmunol.167.1.320
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
TCR and Ig genes are assembled by V(D)J recombination during lymphocyte development. The enhancer and the germline promoter control the accessibility of each locus for the common recombinase activity. In the mouse TCR gamma locus, STAT5 proteins activated by the IL-7R interact with consensus motifs in 5 ' regions of J gamma segments and induce germline transcription. To evaluate the role of STAT5 in controlling the accessibility of the TCR gamma locus, we characterized the germline transcription of human TCR gamma genes and compared it with mouse. We first demonstrated that J gamma -C gamma germline transcripts are induced in a cytokine-dependent human erythroleukemia cell line. STAT consensus motifs are present in 5 ' regions of J-gamma1.1 and J gamma2.1 gene segments, and activated STAT5 binds to these motifs. By using a reporter assay, we showed that the J gamma1.1 germline promoter is transactivated by STAT5 and that mutations in any of the two STAT motifs abrogate this activity. Thus, this study demonstrates that STAT5 induces germline transcription in the TCR gamma locus of both mouse and human and suggests the possibility that this mechanism may play an essential role in controlling the TCR gamma locus accessibility. In addition, STAT motifs are conserved among 5 ' J gamma germline promoters, 3 ' enhancers, and a locus control region-like element, HsA, in both mouse and human TCR gamma loci, indicating the possibility that IL-7R/STAT5 signaling probably controls the locus-wide accessibility through these elements.
引用
收藏
页码:320 / 326
页数:7
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