Electrochemical analysis of electrode-immobilized dehydrogenases in hydrated choline dihydrogen phosphate-type ionic liquid

被引：17

作者：

Fujita, Kyoko ^{[1
]}

Nakamura, Nobuhumi ^{[1
]}

Murata, Kenichi ^{[1
]}

Igarashi, Kiyohiko ^{[2
]}

Samejima, Masahiro ^{[2
]}

Ohno, Hiroyuki ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomat Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

ELECTROCHIMICA ACTA | 2011年 / 56卷 / 20期

基金：

日本学术振兴会;

关键词：

Hydrated; Ionic liquids; Dihydrogen phosphate; Enzyme activity; Biofuel cell; CYTOCHROME-C; LIPASE-B; DISSOLUTION; STABILITY;

D O I：

10.1016/j.electacta.2011.03.107

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A polar and hydrophilic ionic liquid, choline dihydrogen phosphate ([ch][dhp]), has been evaluated here as a medium for a bioelectrocatalytic process for generating cellulosic materials. The [ch][dhp] was mixed with water in proportions that best the competing requirements of solubilization and stabilization of enzymes, solution properties, and process conditions. Fructose dehydrogenase (FDH) and cellobiose dehydrogenase (CDH) were immobilized on gold or carbon nanoparticle-modified electrodes, and their biocatalytic oxidation was detected electrochemically in the hydrated [ch][dhp] (Hy[ch][dhp]). An improvement of the shelf life was found (>3 weeks) of CDH immobilized on the electrode in Hy[ch][dhp]. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：7224 / 7227

页数：4

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