pBIN20:: An improved binary vector for Agrobacterium-mediated transformation

被引：20
|
作者：
Hennegan, KP [1 ]
Danna, KJ [1 ]
机构：
[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期
关键词：
Agrobacterium tumefaciens; binary vector; transformation;
D O I：
10.1023/A:1007444100898
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the construction of a binary vector for Agrobacterium tumefaciens-mediated transformation, pBIN20, which contains a superlinker region located between the left and right Ti border sequences. This vector, derived from pBI121, simplifies the cloning of plant expression cassettes and has been used in our laboratory to create lines of transgenic BY-2 tobacco cells. This new vector contains more than 20 unique restriction sites as well as the nptII selectable marker gene within the Ti-DNA borders.
引用
收藏
页码：129 / 131
页数：3
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