Alternative mRNA splicing of SAP30L regulates its transcriptional repression activity

被引：4

作者：

Korkeamaeki, Hanna ^{[1
]}

Viiri, Keijo ^{[1
]}

Kukkonen, Mari K. ^{[2
]}

Maeki, Markku ^{[1
]}

Lohi, Olli ^{[1
]}

机构：

[1] Univ Tampere, Sch Med, Tampere Univ Hosp, Paediat Res Ctr, Tampere 33520, Finland

[2] Finnish Inst Occupat Hlth, Helsinki 00250, Finland

来源：

FEBS LETTERS | 2008年 / 582卷 / 02期

基金：

芬兰科学院;

关键词：

histone; repression; SAP30L; splicing; transcription;

D O I：

10.1016/j.febslet.2007.11.084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Covalent modification of histones regulates chromatin structure and gene expression. Sin3A mediates the association of histone deacetylase enzymes with a large number of sequence-specific transcriptional repressors. In this study we characterized three novel transcripts of SAP30L, a recently identified Sin3A-associated protein. These splice variants show significant differences in transcriptional repression capabilities and associating histone deacetylase activities. Furthermore, they differ in binding to Sin3A and in subcellular localization when transiently transfected. These data suggest that the transcriptional repression of a Sin3A corepressor complex can be regulated not only by sequence-specific transcriptional repressors, but also by modification of associated proteins, such as SAP30L. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：379 / 384

页数：6

共 11 条

[1] Histone modifications in transcriptional regulation [J].

Berger, SL .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2002, 12 (02) :142-148

[2] Cell cycle-dependent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter [J].

Ferreira, R ;

Naguibneva, I ;

Mathieu, M ;

Ait-Si-Ali, S ;

Robin, P ;

Pritchard, LL ;

Harel-Bellan, A .

EMBO REPORTS, 2001, 2 (09) :794-799

[3]

Kuo MH, 1998, BIOESSAYS, V20, P615, DOI 10.1002/(SICI)1521-1878(199808)20:8<615::AID-BIES4>3.0.CO

[4]

2-H

[5] SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors [J].

Laherty, CD ;

Billin, AN ;

Lavinsky, RM ;

Yochum, GS ;

Bush, AC ;

Sun, JM ;

Mullen, TM ;

Davie, JR ;

Rose, DW ;

Glass, CK ;

Rosenfeld, MG ;

Ayer, DE ;

Eisenman, RN .

MOLECULAR CELL, 1998, 2 (01) :33-42

[6] TGF-β induces the expression of SAP30L, a novel nuclear protein -: art. no. 53 [J].

Lindfors, K ;

Viiri, KM ;

Niittynen, M ;

Heinonen, TYK ;

Mäki, M ;

Kainulainen, H .

BMC GENOMICS, 2003, 4 (1)

[7] Histone sumoylation is associated with transcriptional repression [J].

Shilo, Y ;

Eisenman, RN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (23) :13225-13230

[8] Sin3: a flexible regulator of global gene expression and genome stability [J].

Silverstein, RA ;

Ekwall, K .

CURRENT GENETICS, 2005, 47 (01) :1-17

[9] The language of covalent histone modifications [J].

Strahl, BD ;

Allis, CD .

NATURE, 2000, 403 (6765) :41-45

[10] SAP30L interacts with members of the Sin3A corepressor complex and targets Sin3A to the nucleolus [J].

Viiri, K. M. ;

Korkeamaki, H. ;

Kukkonen, M. K. ;

Nieminen, L. K. ;

Lindfors, K. ;

Peterson, P. ;

Maki, M. ;

Kainulainen, H. ;

Lohi, O. .

NUCLEIC ACIDS RESEARCH, 2006, 34 (11) :3288-3298

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