Astrocyte Differentiation of Human Pluripotent Stem Cells: New Tools for Neurological Disorder Research

被引:92
作者
Chandrasekaran, Abinaya [1 ]
Avci, Hasan X. [1 ,2 ]
Leist, Marcel [3 ]
Kobolak, Julianna [1 ]
Dinnyes, Andras [1 ,4 ]
机构
[1] BioTalentum Ltd, Godollo, Hungary
[2] Univ Szeged, Dept Med Chem, Szeged, Hungary
[3] Univ Konstanz, Fac Math & Sci, Dorenkamp Zbinden Chair, Constance, Germany
[4] Szent Istvan Univ, Mol Anima Biotechnol Lab, Godollo, Hungary
基金
欧盟地平线“2020”;
关键词
astrocyte; glial; central nervous system (CNS); Alzheimer disease (AD); brain pathology; microglia; CNTF; brain damage and repair; FAMILIAL ALZHEIMERS-DISEASE; OUTER SUBVENTRICULAR ZONE; MOTOR-NEURON DEGENERATION; RETINAL GANGLION-CELLS; CENTRAL-NERVOUS-SYSTEM; BLOOD-BRAIN-BARRIER; RADIAL GLIA; PROGENITOR CELLS; DIRECTED DIFFERENTIATION; EFFICIENT GENERATION;
D O I
10.3389/fncel.2016.00215
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Astrocytes have a central role in brain development and function, and so have gained increasing attention over the past two decades. Consequently, our knowledge about their origin, differentiation and function has increased significantly, with new research showing that astrocytes cultured alone or co-cultured with neurons have the potential to improve our understanding of various central nervous system diseases, such as amyotrophic lateral sclerosis, Alzheimer's disease, or Alexander disease. The generation of astrocytes derived from pluripotent stem cells (PSCs) opens up a new area for studying neurologic diseases in vitro; these models could be exploited to identify and validate potential drugs by detecting adverse effects in the early stages of drug development. However, as it is now known that a range of astrocyte populations exist in the brain, it will be important in vitro to develop standardized protocols for the in vitro generation of astrocyte subsets with defined maturity status and phenotypic properties. This will then open new possibilities for co-cultures with neurons and the generation of neural organoids for research purposes. The aim of this review article is to compare and summarize the currently available protocols and their strategies to generate human astrocytes from PSCs. Furthermore, we discuss the potential role of human-induced PSCs derived astrocytes in disease modeling.
引用
收藏
页数:27
相关论文
共 236 条
[31]   GLUTAMATE TRANSPORTERS IN GLIAL PLASMA-MEMBRANES - HIGHLY DIFFERENTIATED LOCALIZATIONS REVEALED BY QUANTITATIVE ULTRASTRUCTURAL IMMUNOCYTOCHEMISTRY [J].
CHAUDHRY, FA ;
LEHRE, KP ;
CAMPAGNE, MV ;
OTTERSEN, OP ;
DANBOLT, NC ;
STORMMATHISEN, J .
NEURON, 1995, 15 (03) :711-720
[32]   Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats [J].
Chen, Haw-Wen ;
Huang, Chin-Shiu ;
Li, Chien-Chun ;
Lin, Ai-Hsuan ;
Huang, Yu-Ju ;
Wang, Tsu-Shing ;
Yao, Hsien-Tsung ;
Lii, Chong-Kuei .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2014, 280 (01) :1-9
[33]   Rapid generation of mature hepatocyte-like cells from human induced pluripotent stem cells by an efficient three-step protocol [J].
Chen, Yu-Fan ;
Tseng, Chien-Yu ;
Wang, Hsei-Wei ;
Kuo, Hung-Chih ;
Yang, Vincent W. ;
Lee, Oscar K. .
HEPATOLOGY, 2012, 55 (04) :1193-1203
[34]   Patient-Specific Induced Pluripotent Stem Cells for SOD1-Associated Amyotrophic Lateral Sclerosis Pathogenesis Studies [J].
Chestkov, I. V. ;
Vasilieva, E. A. ;
Illarioshkin, S. N. ;
Lagarkova, M. A. ;
Kiselev, S. L. .
ACTA NATURAE, 2014, 6 (01) :54-60
[35]   Motor neuron degeneration promotes neural progenitor cell proliferation, migration, and neurogenesis in the spinal cords of amyotrophic lateral sclerosis mice [J].
Chi, LY ;
Ke, Y ;
Luo, C ;
Li, BL ;
Gozal, D ;
Kalyanaraman, B ;
Liu, R .
STEM CELLS, 2006, 24 (01) :34-43
[36]   Emerging roles of astrocytes in neural circuit development [J].
Clarke, Laura E. ;
Barres, Ben A. .
NATURE REVIEWS NEUROSCIENCE, 2013, 14 (05) :311-321
[37]   LONG, INTERLAMINAR ASTROGLIAL CELL PROCESSES IN THE CORTEX OF ADULT MONKEYS [J].
COLOMBO, JA ;
YANEZ, A ;
PUISSANT, V ;
LIPINA, S .
JOURNAL OF NEUROSCIENCE RESEARCH, 1995, 40 (04) :551-556
[38]   Astrocytic regulation of glutamate homeostasis in epilepsy [J].
Coulter, Douglas A. ;
Eid, Tore .
GLIA, 2012, 60 (08) :1215-1226
[39]   The organic cation transporter-3 is a pivotal modulator of neurodegeneration in the nigrostriatal dopaminergic pathway [J].
Cui, Mei ;
Aras, Radha ;
Christian, Whitney V. ;
Rappold, Phillip M. ;
Hatwar, Mamata ;
Panza, Joseph ;
Jackson-Lewis, Vernice ;
Javitch, Jonathan A. ;
Ballatori, Nazzareno ;
Przedborski, Serge ;
Tieu, Kim .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (19) :8043-8048
[40]   Alzheimer's disease drug-development pipeline: few candidates, frequent failures [J].
Cummings, Jeffrey L. ;
Morstorf, Travis ;
Zhong, Kate .
ALZHEIMERS RESEARCH & THERAPY, 2014, 6 (04)