Diffusional mobility of Golgi proteins in membranes of living cells

被引:403
作者
Cole, NB
Smith, CL
Sciaky, N
Terasaki, M
Edidin, M
LippincottSchwartz, J
机构
[1] NICHHD, CELL BIOL & METAB BRANCH, NIH, BETHESDA, MD 20892 USA
[2] NINCDS, NIH, BETHESDA, MD 20892 USA
[3] JOHNS HOPKINS UNIV, DEPT BIOL, BALTIMORE, MD 21218 USA
[4] UNIV CONNECTICUT, CTR HLTH, DEPT PHYSIOL, FARMINGTON, CT 06032 USA
关键词
D O I
10.1126/science.273.5276.797
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mechanism by which Golgi membrane proteins are retained with in the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 x 10(-9) square centimeters per second to 5 x 10(-9) square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.
引用
收藏
页码:797 / 801
页数:5
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