Polarized illumination coded structured illumination microscopy (picoSIM): experimental results

被引：4

作者：

Appelt, Daniel ^{[1
,5
]}

Ehler, Elisabeth ^{[1
]}

Mukherjee, Sapna Shukla ^{[2
,3
,4
]}

Heintzmann, Rainer ^{[1
,2
,3
,4
]}

Wicker, Kai ^{[2
,3
,4
,6
]}

机构：

[1] Kings Coll London, Randall Div Cell & Mol Biophys, Guys Campus, London SE1 1UL, England

[2] Leibniz Inst Photon Technol, Albert Einstein Str 9, D-07745 Jena, Germany

[3] Friedrich Schiller Univ Jena, Inst Phys Chem, Helmholtzweg 4, D-07743 Jena, Germany

[4] Friedrich Schiller Univ Jena, Abbe Ctr Photon, Helmholtzweg 4, D-07743 Jena, Germany

[5] Arthrex GmbH, Erwin Hielscher Str 9, D-81249 Munich, Germany

[6] Carl Zeiss, ZEISS Innovat Hub Dresden, Else Kroner Fresenius Ctr Digital Hlth, Fetscherstr 74, D-01307 Dresden, Germany

来源：

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES | 2022年 / 380卷 / 2220期

关键词：

resolution; super-resolution; structured illumination; fluorescence; sectioning; RESOLUTION; EXCITATION; PHASE;

D O I：

10.1098/rsta.2021.0193

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

引用

页数：12

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