The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple

被引：58

作者：

Peng, Qian ^{[1
,2
]}

Cai, Yaming ^{[1
,2
]}

Lai, Enhui ^{[1
,2
]}

Nakamura, Masayoshi ^{[3
]}

Liao, Liao ^{[1
,4
]}

Zheng, Beibei ^{[1
,4
]}

Ogutu, Collins ^{[1
,2
]}

Cherono, Sylvia ^{[1
,2
]}

Han, Yuepeng ^{[1
,4
,5
]}

机构：

[1] Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China

[2] Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China

[3] Nagoya Univ, Inst Transformat Biomol, Nagoya, Aichi, Japan

[4] Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Wuhan 430074, Peoples R China

[5] Chinese Acad Sci, Sino African Joint Res Ctr, Wuhan 430074, Peoples R China

来源：

BMC PLANT BIOLOGY | 2020年 / 20卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Malus x domestica; Gene-tagged marker; SUT; Tonoplast localization; Fruit sweetness; FUNCTIONAL-CHARACTERIZATION; QUALITY TRAITS; SIEVE ELEMENTS; GRAPE BERRY; PHLOEM; ARABIDOPSIS; TONOPLAST; OVEREXPRESSION; IDENTIFICATION; EXPRESSION;

D O I：

10.1186/s12870-020-02406-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background Sugar content is an important determinant of fruit sweetness, but details on the complex molecular mechanism underlying fruit sugar accumulation remain scarce. Here, we report the role of sucrose transporter (SUT) family in regulating fruit sugar accumulation in apple. Results Gene-tagged markers were developed to conduct candidate gene-based association study, and an SUT4 member MdSUT4.1 was found to be significantly associated with fruit sugar accumulation. MdSUT4.1 encodes a tonoplast localized protein and its expression level had a negative correlation with fruit sugar content. Overexpression of MdSUT4.1 in strawberry and apple callus had an overall negative impact on sugar accumulation, suggesting that it functions to remobilize sugar out of the vacuole. In addition, MdSUT4.1 is located on chromosomal region harboring a previously reported QTL for sugar content, suggesting that it is a candidate gene for fruit sugar accumulation in apple. Conclusions MdSUT4.1 is involved in the regulation of fruit sugar accumulation in apple. This study is not only helpful for understanding the complex mechanism of fruit sugar accumulation, but it also provides molecular tools for genetic improvement of fruit quality in breeding programs of apple.

引用

页数：14

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