Modulation of Human Immune Responses by Bovine Interleukin-10

被引：23

作者：

den Hartog, Gerco ^{[1
]}

Savelkoul, Huub F. J. ^{[1
]}

Schoemaker, Ruud ^{[2
]}

Tijhaar, Edwin ^{[1
]}

Westphal, Adrie H. ^{[3
]}

de Ruiter, Talitha ^{[2
]}

van de Weg-Schrijver, Elise ^{[2
]}

van Neerven, R. J. Joost ^{[2
]}

机构：

[1] Wageningen Univ, Cell Biol & Immunol Grp, Wageningen, Netherlands

[2] Royal FrieslandCampina Res, Deventer, Netherlands

[3] Wageningen Univ, Biochem Lab, Wageningen, Netherlands

来源：

PLOS ONE | 2011年 / 6卷 / 03期

关键词：

T-CELL PROLIFERATION; DENDRITIC CELLS; IFN-GAMMA; NECROTIZING ENTEROCOLITIS; SUBLINGUAL IMMUNOTHERAPY; CYTOKINE PRODUCTION; INTERFERON-GAMMA; AUTOCRINE IL-10; HUMAN-MILK; TGF-BETA;

D O I：

10.1371/journal.pone.0018188

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.

引用

页数：10

共 64 条

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