Development and characterization of an in vitro alpha radiation exposure system

被引：16
作者：
Beaton, Lindsay A. [3 ]
Burn, Trevor A. [3 ]
Stocki, Trevor J. [2 ]
Chauhan, Vinita [1 ]
Wilkins, Ruth C. [1 ]
机构：
[1] Hlth Canada, Consumer & Clin Radiat Protect Bur, Ottawa, ON K1A 0L2, Canada
[2] Hlth Canada, Radiat Protect Bur, Ottawa, ON K1A 0L2, Canada
[3] Carleton Univ, Dept Phys, Ottawa, ON K1S 5B6, Canada
来源：
PHYSICS IN MEDICINE AND BIOLOGY | 2011年 / 56卷 / 12期
关键词：
MONTE-CARLO-SIMULATION; PARTICLE IRRADIATOR; CELLS; DESIGN; RADON; INDUCTION; TOOLKIT; CANCER; DEVICE;
D O I：
10.1088/0031-9155/56/12/012
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A simple in vitro alpha radiation exposure system (ARES) was designed to study the biological effects of alpha particle radiation. The ARES consists of six Am-241 electroplated stainless steel discs with activities averaging 66 kBq and Mylar-based culture dishes to allow the transmission of alpha particles. The dosimetry of the exposure system was calculated using the GEANT4 Monte Carlo simulation toolkit with the source code adapted from the open-source Microbeam example. The average dose rate and linear energy transfer of the system was simulated to be 0.98 +/- 0.01 (statistical)(-0.00)(+0.18) (systematic) Gy h(-1) and 127.4 +/- 0.4 (statistical)(-0)(+23) (systematic) keV mu m(-1), respectively. The system was characterized by a comparison of the survival curves of gamma and alpha irradiated cell lines which showed a relative biological effectiveness of 6.3. This is in good agreement with values obtained using other published alpha particle exposure systems. Results show that the ARES provides a simple, cost-effective exposure platform for research into the biological effects of alpha particle radiation using in vitro modelling of cell cultures.
引用
收藏
页码：3645 / 3658
页数：14
相关论文
共 32 条
[1]   GEANT4-a simulation toolkit [J].
Agostinelli, S ;
Allison, J ;
Amako, K ;
Apostolakis, J ;
Araujo, H ;
Arce, P ;
Asai, M ;
Axen, D ;
Banerjee, S ;
Barrand, G ;
Behner, F ;
Bellagamba, L ;
Boudreau, J ;
Broglia, L ;
Brunengo, A ;
Burkhardt, H ;
Chauvie, S ;
Chuma, J ;
Chytracek, R ;
Cooperman, G ;
Cosmo, G ;
Degtyarenko, P ;
Dell'Acqua, A ;
Depaola, G ;
Dietrich, D ;
Enami, R ;
Feliciello, A ;
Ferguson, C ;
Fesefeldt, H ;
Folger, G ;
Foppiano, F ;
Forti, A ;
Garelli, S ;
Giani, S ;
Giannitrapani, R ;
Gibin, D ;
Cadenas, JJG ;
González, I ;
Abril, GG ;
Greeniaus, G ;
Greiner, W ;
Grichine, V ;
Grossheim, A ;
Guatelli, S ;
Gumplinger, P ;
Hamatsu, R ;
Hashimoto, K ;
Hasui, H ;
Heikkinen, A ;
Howard, A .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2003, 506 (03) :250-303
[2]   Effect of linear energy transfer (LET) on the complexity of α-particle-induced chromosome aberrations in human CD34+ cells [J].
Anderson, Rhona M. ;
Stevens, David L. .
RADIATION RESEARCH, 2007, 167 (05) :541-550
[3]   Effects of low and high LET radiations on bystander human lung fibroblast cell survival [J].
Baskar, Rajamanickam ;
Balajee, Adayabalam S. ;
Geard, Charles R. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2007, 83 (08) :551-559
[4]   A preliminary radon map for Canada according to health region [J].
Chen, Jing ;
Jiang, Huixia ;
Tracy, Bliss L. ;
Zielinski, Jan M. .
RADIATION PROTECTION DOSIMETRY, 2008, 130 (01) :92-94
[5]   Relative biological effectiveness of the α-particle emitter 211At for double-strand break induction in human fibroblasts [J].
Claesson, Anna Kristina ;
Stenerlow, Bo ;
Jacobsson, Lars ;
Elmroth, Kecke .
RADIATION RESEARCH, 2007, 167 (03) :312-318
[6]   Cancer Therapy with Alpha-Emitters Labeled Peptides [J].
Dadachova, Ekaterina .
SEMINARS IN NUCLEAR MEDICINE, 2010, 40 (03) :204-208
[7]   Monte-Carlo study of energy deposition by heavy charged particles in sub-cellular volumes [J].
Emfietzoglou, D. ;
Papamichael, G. ;
Pathak, A. ;
Fotopoulos, A. ;
Nikjoo, H. .
RADIATION PROTECTION DOSIMETRY, 2007, 126 (1-4) :457-462
[8]   An Alpha-Particle Irradiator for Radiobiological Research and its Implementation for Bystander Effect Studies [J].
Esposito, Giuseppe ;
Antonelli, Francesca ;
Belli, Mauro ;
Campa, Alessandro ;
Simone, Glustina ;
Sorrentino, Eugenio ;
Tabocchini, Maria Antonella .
RADIATION RESEARCH, 2009, 172 (05) :632-642
[9]  
Firestone R.B., 1996, Table of Isotopes, Veighth
[10]   Calculated depth-dose distributions for H+ and He+ beams in liquid water [J].
Garcia-Molina, Rafael ;
Abril, Isabel ;
Denton, Cristian D. ;
Heredia-Avalos, Santiago ;
Kyriakou, Ioanna ;
Emfietzoglou, Dimitris .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2009, 267 (16) :2647-2652
1234