Self-aligned myofibers in 3D bioprinted extracellular matrix-based construct accelerate skeletal muscle function restoration

被引:43
作者
Lee, Hyeongjin [1 ]
Kim, WonJin [1 ,2 ]
Lee, JiUn [1 ,3 ]
Park, Kyung Soon [1 ]
Yoo, James J. [1 ]
Atala, Anthony [1 ]
Kim, Geun Hyung [1 ,2 ,4 ]
Lee, Sang Jin [1 ]
机构
[1] Wake Forest Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC 27157 USA
[2] Sungkyunkwan Univ SKKU, Coll Biotechnol & Bioengn, Dept Biomechatron Engn, Suwon 16419, South Korea
[3] Chonnam Natl Univ, Dept Orthoped Surg, Bitgoeul Hosp, Kwangju 61748, South Korea
[4] Sungkyunkwan Univ, Biomed Inst Convergence SKKU BICS, Suwon 16419, South Korea
基金
美国国家卫生研究院; 新加坡国家研究基金会;
关键词
TISSUE ENGINEERING STRATEGIES; VOLUMETRIC MUSCLE; BIOMATERIALS; REGENERATION; FABRICATION; SCAFFOLDS; MODEL;
D O I
10.1063/5.0039639
中图分类号
O59 [应用物理学];
学科分类号
摘要
To achieve rapid skeletal muscle function restoration, many attempts have been made to bioengineer functional muscle constructs by employing physical, biochemical, or biological cues. Here, we develop a self-aligned skeletal muscle construct by printing a photo-crosslinkable skeletal muscle extracellular matrix-derived bioink together with poly(vinyl alcohol) that contains human muscle progenitor cells. To induce the self-alignment of human muscle progenitor cells, in situ uniaxially aligned micro-topographical structure in the printed constructs is created by a fibrillation/leaching of poly(vinyl alcohol) after the printing process. The in vitro results demonstrate that the synergistic effect of tissue-specific biochemical signals (obtained from the skeletal muscle extracellular matrix-derived bioink) and topographical cues [obtained from the poly(vinyl alcohol) fibrillation] improves the myogenic differentiation of the printed human muscle progenitor cells with cellular alignment. Moreover, this self-aligned muscle construct shows the accelerated integration with neural networks and vascular ingrowth in vivo, resulting in rapid restoration of muscle function. We demonstrate that combined biochemical and topographic cues on the 3D bioprinted skeletal muscle constructs can effectively reconstruct the extensive muscle defect injuries.
引用
收藏
页数:14
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