Further evaluation of the biological activity of the unique gonadotropin-releasing hormone peptide in the guinea pig brain

被引：5
|
作者：
Gao, Chang-Qing [1 ,2 ,3 ]
Fraeyman, Norbert [3 ]
Eertmans, Frank [3 ]
Dhooge, Willem [3 ]
Kaufman, Jean-Marc [3 ]
机构：
[1] Cent S Univ, XiangYa Hosp 3, Res Ctr Med Sci, Changsha 410013, Hunan, Peoples R China
[2] Cent S Univ, XiangYa Hosp 3, Dept Radiol, Changsha 410013, Hunan, Peoples R China
[3] Ghent Univ Hosp, Dept Endocrinol, B-9000 Ghent, Belgium
来源：
NEUROSCIENCE LETTERS | 2011年 / 487卷 / 02期
基金：
湖南省自然科学基金;
关键词：
Guinea pig; GnRH; Bioactivity; Proteolytic degradation; LUTEINIZING-HORMONE; ENZYMATIC DEGRADATION; LHRH; ANALOGS; PITUITARY; CLEAVAGE; RECEPTOR; INVITRO; GNRH;
D O I：
10.1016/j.neulet.2010.10.031
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
In this study we compared the biological activity of a unique form of gonadotropin-releasing hormone (GnRH) in the brain of the guinea pig (gpGnRH) with mammalian GnRH (mGnRH). In gpGnRH, the highly conserved histidine in position 2 (His(2)) and leucine in position 7 (Leu(7)) are substituted by tyrosine and valine, respectively. The gpGnRH was less potent than mGnRH in stimulating the release of luteinizing hormone (LH) in vivo in the guinea pig and displayed only low activity in the rat. The gpGnRH was more rapidly degraded by serum proteolytic enzymes than mGnRH. It is concluded that gpGnRH displays lower biological activity than mGnRH in both rat and guinea pig, which may be due in part to its greater susceptibility to proteolytic degradation besides differences in receptor affinity and/or activation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：246 / 249
页数：4
相关论文
共 50 条
  • [21] Cloning of corticotropin-releasing hormone (CRH) precursor cDNA and immunohistochemical detection of CRH peptide in the brain of the Japanese eel, paying special attention to gonadotropin-releasing hormone
    Masafumi Amano
    Nanami Mizusawa
    Kataaki Okubo
    Noriko Amiya
    Kanta Mizusawa
    Hiroaki Chiba
    Naoyuki Yamamoto
    Akiyoshi Takahashi
    Cell and Tissue Research, 2014, 356 : 243 - 251
  • [22] Glucocorticoids affect gonadotropin-releasing hormone immunoreactivity in musk shrew brain
    Schiml-Webb, PA
    Temple, JL
    Rissman, EF
    GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2001, 123 (02) : 163 - 169
  • [23] Discovery of a Lead Brain-Penetrating Gonadotropin-Releasing Hormone Receptor Antagonist with Saturable Binding in Brain
    Bekker, Roberto B. W.
    Fjellaksel, Richard
    Hjornevik, Trine
    Nuruddin, Syed
    Rafique, Waqas
    Hansen, Jorn H.
    Sundset, Rune
    Haraldsen, Ira H.
    Riss, Patrick J.
    CHEMMEDCHEM, 2020, 15 (17) : 1624 - 1628
  • [24] Orexin A Suppresses Gonadotropin-Releasing Hormone (GnRH) Neuron Activity in the Mouse
    Gaskins, Garrett T.
    Moenter, Suzanne M.
    ENDOCRINOLOGY, 2012, 153 (08) : 3850 - 3860
  • [25] Evaluation of plasmid delivery by electroporation as a means of increasing gonadotropin-releasing hormone production in stallions
    Storer, W. A.
    Thompson, D. L., Jr.
    Bondioli, K. R.
    Godke, R. A.
    Khan, A. S.
    Brown, P. A.
    Draghia-Akil, R.
    JOURNAL OF EQUINE VETERINARY SCIENCE, 2008, 28 (03) : 149 - 155
  • [26] CHANGES IN GONADOTROPIN-RELEASING HORMONE AND GONADOTROPIN-RELEASING HORMONE RECEPTOR GENE EXPRESSION AFTER AN INCREASE IN CARBON MONOXIDE CONCENTRATION IN THE CAVERNOUS SINUS OF MALE WILD BOAR AND PIG CROSSBREED
    Romerowicz-Misielak, M.
    Tabecka-Lonczynska, A.
    Koziol, K.
    Gilun, P.
    Stefanczyk-Krzymowska, S.
    Och, W.
    Koziorowski, M.
    JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2016, 67 (03): : 431 - 442
  • [27] Age-related changes in gonadotropin-releasing hormone (GnRH) splice variants in mouse brain
    Gautam, Pooja
    Ajit, Kamal
    Das, Moitreyi
    Taliyan, Rajeev
    Roy, Ramaballav
    Banerjee, Arnab
    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY, 2023, 339 (02) : 193 - 209
  • [28] A novel indium-111-labeled gonadotropin-releasing hormone peptide for human prostate cancer imaging
    Guo, Haixun
    Gallazzi, Fabio
    Sklar, Larry A.
    Miao, Yubin
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2011, 21 (18) : 5184 - 5187
  • [29] Interaction between alpha-fetoprotein gene and gonadotropin-releasing hormone and effects on brain sexual differentation: Molecular function and biological process
    Wiwanitkit, Viroj
    SEXUALITY AND DISABILITY, 2006, 24 (03) : 169 - 173
  • [30] Interaction between Alpha-Fetoprotein Gene and Gonadotropin-Releasing Hormone and Effects on Brain Sexual Differentation: Molecular Function and Biological Process
    Viroj Wiwanitkit
    Sexuality and Disability, 2006, 24 : 169 - 173
12345