NKp80 Defines a Critical Step during Human Natural Killer Cell Development

被引：94

作者：

Freud, Aharon G. ^{[1
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Keller, Karen A. ^{[1
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Scoville, Steven D. ^{[2
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Mundy-Bosse, Bethany L. ^{[4
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Cheng, Stephanie ^{[1
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Youssef, Youssef ^{[1
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Hughes, Tiffany ^{[4
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Zhang, Xiaoli ^{[5
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Mo, Xiaokui ^{[5
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Porcu, Pierluigi ^{[4
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Baiocchi, Robert A. ^{[4
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Yu, Jianhua ^{[4
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Carson, William E., III ^{[6
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Caligiuri, Michael A. ^{[4
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机构：

[1] Ohio State Univ, Dept Pathol, Columbus, OH 43210 USA

[2] Ohio State Univ, Med Scientist Training Program, Columbus, OH 43210 USA

[3] Ohio State Univ, Biomed Sci Grad Program, Columbus, OH 43210 USA

[4] Ohio State Univ, Dept Internal Med, Div Hematol, Columbus, OH 43210 USA

[5] Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USA

[6] Ohio State Univ, Dept Surg, Div Surg Oncol, Columbus, OH 43210 USA

[7] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA

[8] Ohio State Univ, James Canc Hosp, Columbus, OH 43210 USA

[9] Ohio State Univ, Solove Res Inst, Columbus, OH 43210 USA

来源：

CELL REPORTS | 2016年 / 16卷 / 02期

关键词：

INNATE LYMPHOID-CELLS; NK-CELL; DENDRITIC CELLS; CLASS-I; DIFFERENTIATION; SUBSET; EXPRESSION; DISTINCT; RECEPTOR; TISSUES;

D O I：

10.1016/j.celrep.2016.05.095

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)(-)CD34(-)CD117(+/-)CD94(+)CD16(-) "stage 4'' subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80:NKp80(-) (stage "4a'') and NKp80(+) (stage "4b''). Whereas stage 4b cells expressed more of the transcription factors T-BET and EOMES, produced interferon-gamma, and were cytotoxic, stage 4a cells expressed more of the transcription factors ROR gamma t and AHR and produced interleukin-22, similar to SLT Lin(-)CD34(-)CD117(+)CD94(-)CD16(-) "stage 3'' cells, whose phenotype overlaps with that of group 3 innate lymphoid cells (ILC3s). Co-culture with dendritic cells or transplantation into immunodeficient mice produced mature NK cells from stage 3 and stage 4a populations. These data identify NKp80 as a marker of NK cell maturity in SLTs and support a model of human NK cell development through a stage 4a intermediate with ILC3-associated features.

引用

页码：379 / 391

页数：13

共 48 条

[1] Lineage relationships of human interleukin-22-producing CD56+ RORγt+ innate lymphoid cells and conventional natural killer cells [J].