Asymmetric reduction of acetophenone with calcium-alginate-entrapped Baker's yeast in organic solvents

Baker's yeast cells entrapped in alginate beads are shown to catalyze reactions in organic solvents when a cofactor regeneration scheme is implemented. This study focused on the reduction of acetophenone to 1-phenylethanol, using baker's yeast as well as a cosubstrate to regenerate the cofactor. The product is a chiral alcohol, and it was desired to maintain a high enantiomeric excess. The effects of parameters such as the addition of a cosubstrate, water content, fermentation time, buffer pH, and bead diameter have been investigated Such a general process may be quite useful when single enantiomers are needed, as well as for the production of other chemicals.