Development of a sutureless dural substitute from Bombyx mori silk fibroin

被引:23
作者
Flanagan, Kelly E. [1 ]
Tien, Lee W. [1 ]
Elia, Roberto [1 ]
Wu, Julian [2 ]
Kaplan, David [1 ]
机构
[1] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
[2] Tufts Med Ctr, Dept Neurosurg, Boston, MA USA
关键词
dural substitute; silk fibroin; electrospinning; biodegradable composite; tissue sealant; FIBROBLASTS; ADHESION;
D O I
10.1002/jbm.b.33217
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Silk solvent casting, electrospinning, and electrogelation techniques were used to create a biodegradable, biocompatible silk fibroin dural substitute. The all-silk system was designed and produced to improve on currently available materials, grafts and tissue sealants used for dural closure in neurosurgery. The silk biomaterial was successfully fabricated as a dual layer adhesive system designed to seal durotomies while also functioning as a dural regeneration scaffold. The mechanical characteristics, biocompatibility, biodegradability, and hydrodynamic sealing capability of the material were evaluated. Results showed that the biomaterial was biocompatible with neural cells and fibroblasts, had mechanical properties mimicking the natural dura, was biodegradable with controllable degradation, and was able to seal against a hydrodynamic pressure of 205 mmHg, which greatly exceeds the maximum cerebrospinal fluid pressure seen in both cranial and spinal dural closures of 50 mmHg. Based on its design and experimental results, the adhesive silk dual layer composite biomaterial shows potential as a sutureless dural repair system that would improve on current dural closure techniques. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 485-494, 2015.
引用
收藏
页码:485 / 494
页数:10
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