Bystander Effect of Conditioned Medium from Low and High Doses of γ-Irradiated Human Leukemic Cells on Normal Lymphocytes and Cancer Cells

被引：7

作者：

Pandey, B. N. ^{[1
]}

Kumar, A. ^{[1
]}

Ali, M. ^{[1
]}

Mishra, K. P. ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Radiat Biol & Hlth Sci Div, Bombay 400085, Maharashtra, India

来源：

JOURNAL OF ENVIRONMENTAL PATHOLOGY TOXICOLOGY AND ONCOLOGY | 2011年 / 30卷 / 04期

关键词：

Radiation induced bystander effect; apoptosis; reactive oxygen species; mitochondrial membrane potential; low and high dose; diffusible factors; lymphocytes and cancer cells; HYPER-RADIOSENSITIVITY; GLIOMA-CELLS; NONIRRADIATED CELLS; NITRIC-OXIDE; RADIATION; APOPTOSIS; HYPERSENSITIVITY; RADIOTHERAPY; STRESS; COMMUNICATION;

D O I：

10.1615/JEnvironPatholToxicolOncol.v30.i4.60

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The present study investigated the effect of irradiated conditioned medium (ICM), obtained from human promyelocytic leukemic cells (HL-60) gamma-irradiated either with low-dose (5 cGy; ICM-L) or high-dose radiation (1 Gy; ICM-H) on unirradiated HL-60 and normal human blood lymphocytes. A dose-dependent increase in apoptosis was observed in HL-60 cells cultured in ICM obtained after different doses of irradiation (0.5-10 cGy), which showed saturation in ICM from 5 cGy. Furthermore, ICM-L and ICM-H induced differential apoptotic responses in unirradiated HL-60 cells and lymphocytes. Apoptosis in lymphocytes cultured in ICM-H was significantly higher than when cells were cultured in unirradiated conditioned medium or ICM-L. Furthermore, induction of apoptosis in bystander HL-60 cells and lymphocytes cultured in ICM-L/ICM-H seems to be associated with an increase in intracellular reactive oxygen species (ROSs) and alterations in mitochondrial membrane potential. These results suggest that differential bystander responses from irradiated cancer cells toward cancer and normal cells depending on the applied dose and/or dose rate of radiation..

引用

页码：333 / 340

页数：8

共 33 条

[1]

Amundson SA, 2003, MOL CANCER RES, V1, P445

[2] Stress signaling frorn irradiated to non-irradiated cells [J].

Azzam, EI ;

de Toledo, SM ;

Little, JB .

CURRENT CANCER DRUG TARGETS, 2004, 4 (01) :53-64

[3] Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect [J].

Azzam, EI ;

de Toledo, SM ;

Little, JB .

ONCOGENE, 2003, 22 (45) :7050-7057

[4] Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from α-particle irradiated to nonirradiated cells [J].

Azzam, EI ;

de Toledo, SM ;

Little, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) :473-478

[5] Increased mitochondrial biogenesis in primary leukemia cells: the role of endogenous nitric oxide and impact on sensitivity to fludarabine [J].

Carew, JS ;

Nawrocki, ST ;

Xu, RH ;

Dunner, K ;

McConkey, DJ ;

Wierda, WG ;

Keating, MJ ;

Huang, P .

LEUKEMIA, 2004, 18 (12) :1934-1940

[6]

Enns L, 2004, MOL CANCER RES, V2, P557

[7] Estimating the optimal utilization rates of radiotherapy for hematologic malignancies from a review of the evidence - Part II - Leukemia and myeloma [J].

Featherstone, C ;

Delaney, G ;

Jacob, S ;

Barton, M .

CANCER, 2005, 103 (02) :393-401

[8]

Giaccia, 2006, RADIOBIOLOGY RADIOLO, VVol 6

[9] The central executioners of apoptosis: caspases or mitochondria? [J].

Green, D ;

Kroemer, G .

TRENDS IN CELL BIOLOGY, 1998, 8 (07) :267-271

[10] Mitochondria and apoptosis [J].

Green, DR ;

Reed, JC .

SCIENCE, 1998, 281 (5381) :1309-1312

← 1 2 3 4 →