Bacillus cereus-induced food-borne outbreaks in France, 2007 to 2014: epidemiology and genetic characterisation

被引：110

作者：

Glasset, B. ^{[1
,2
]}

Herbin, S. ^{[1
]}

Guillier, L. ^{[1
]}

Cadel-Six, S. ^{[1
]}

Vignaud, M. ^{[1
]}

Grout, J. ^{[1
]}

Pairaud, S. ^{[1
]}

Michel, V. ^{[3
]}

Hennekinne, J. ^{[1
]}

Ramarao, N. ^{[2
]}

Brisabois, A. ^{[1
]}

机构：

[1] Univ Paris Est, ANSES, Lab Food Safety, Maisons Alfort, France

[2] Univ Paris Saclay, AgroParisTech, INRA, Micalis Inst, Jouy En Josas, France

[3] ACTALIA, La Roche Sur Foron, France

来源：

EUROSURVEILLANCE | 2016年 / 21卷 / 48期

关键词：

ENTEROTOXIN PRODUCTION; STRAINS; IDENTIFICATION; PREVALENCE; FAMILY;

D O I：

10.2807/1560-7917.ES.2016.21.48.30413

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of this study was to identify and characterise Bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014. Starchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. Incubation period was significantly shorter for emetic strains compared with diarrhoeal strains A sub-panel of 149 strains strictly associated to 74 FBOs and selected on Coliphage M13-PCR pattern, was studied for detection of the genes encoding cereulide, diarrhoeic toxins (Nhe, Hbl, CytK1 and CytK2) and haemolysin (HlyII), as well as panC phylogenetic classification. This clustered the strains into 12 genetic signatures (GSs) highlighting the virulence potential of each strain. GS1 (nhe genes only) and GS2 (nhe, hbl and cytK2), were the most prevalent GS and may have a large impact on human health as they were present in 28% and 31% of FBOs, respectively. Our study provides a convenient molecular scheme for characterisation of B. cereus strains responsible for FBOs in order to improve the monitoring and investigation of B. cereus-induced FBOs, assess emerging clusters and diversity of strains.

引用

页码：18 / 28

页数：11

共 33 条

[1] Production of Bacillus cereus emetic toxin (cereulide) in various foods [J].

Agata, N ;

Ohta, M ;

Yokoyama, K .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2002, 73 (01) :23-27

[2]

[Anonymous], 2015, WHO Estimates of the Global Burden of Foodborne Diseases

[3]

[Anonymous], B EPIDEMIOLOGIQUE HE

[4] IDENTIFICATION OF HEMOLYSIN BL-PRODUCING BACILLUS-CEREUS ISOLATES BY A DISCONTINUOUS HEMOLYTIC PATTERN IN BLOOD AGAR [J].

BEECHER, DJ ;

WONG, ACL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (05) :1646-1651

[5]

Boelaert F, 2015, EFSA J, V13, DOI [10.2903/j.efsa.2015.3991, 10.2903/j.efsa.2015.4302]

[6] InhA1, NprA, and HlyII as Candidates for Markers To Differentiate Pathogenic from Nonpathogenic Bacillus cereus Strains [J].

Cadot, Celine ;

Tran, Seav-Ly ;

Vignaud, Marie-Leone ;

De Buyser, Marie-Laure ;

Kolsto, Anne-Brit ;

Brisabois, Anne ;

Nguyen-The, Christophe ;

Lereclus, Didier ;

Guinebretiere, Marie-Helene ;

Ramarao, Nalini .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (04) :1358-1365

[7] Enterotoxin Production by Bacillus cereus Under Gastrointestinal Conditions and Their Immunological Detection by Commercially Available Kits [J].

Ceuppens, Siele ;

Rajkovic, Andreja ;

Hamelink, Stefanie ;

Van de Wiele, Tom ;

Boon, Nico ;

Uyttendaele, Mieke .

FOODBORNE PATHOGENS AND DISEASE, 2012, 9 (12) :1130-1136

[8] Prevalence, characterization and growth of Bacillus cereus in commercial cooked chilled foods containing vegetables [J].

Choma, C ;

Guinebretière, MH ;

Carlin, F ;

Schmitt, P ;

Velge, P ;

Granum, PE ;

Nguyen-The, C .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (04) :617-625

[9] Prevalence, phenotypic traits and molecular characterization of emetic toxin-producing Bacillus cereus strains isolated from human stools in Korea [J].

Chon, J. W. ;

Kim, J. H. ;

Lee, S. J. ;

Hyeon, J. Y. ;

Song, K. Y. ;

Park, C. ;

Seo, K. -H. .

JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (05) :1042-1049

[10] Isolation of Bacillus cytotoxicus from various commercial potato products [J].

Contzen, Matthias ;

Hailer, Mandy ;

Rau, Joerg .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2014, 174 :19-22

← 1 2 3 4 →