Expression of soybean β-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants

被引：30

作者：

Nakamura, Y

Sawada, H

Kobayashi, S

Nakajima, I

Yoshikawa, M

机构：

[1] Natl Inst Fruit Tree Sci, Persimmon & Grape Res Ctr, Akitsu, Hiroshima 7292494, Japan

[2] Natl Inst Agr Environm Sci, Ibaraki, Osaka 3058604, Japan

[3] Hokkaido Univ, Fac Sci, Dept Bot, Sapporo, Hokkaido 060, Japan

来源：

PLANT CELL REPORTS | 1999年 / 18卷 / 7-8期

关键词：

Agrobacterium; kiwifruit; beta-1,3-endoglucanase; transformation; disease tolerance;

D O I：

10.1007/s002990050616

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Kiwifruit was transformed with a soybean beta-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.

引用

页码：527 / 532

页数：6