T Cells Chemotaxis Migration Studies with a Multi-Channel Microfluidic Device

被引：6

作者：

Liu, Yang ^{[1
]}

Ren, Xiaoou ^{[1
]}

Wu, Jiandong ^{[2
]}

Wilkins, John A. ^{[3
,4
]}

Lin, Francis ^{[1
]}

机构：

[1] Univ Manitoba, Dept Phys & Astron, 30A Sifton Rd,301 Allen Bldg, Winnipeg, MB R3T 2N2, Canada

[2] Chinese Acad Sci, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[3] Univ Manitoba, Manitoba Ctr Prote & Syst Biol, 799 JBRC,715 McDermot Ave, Winnipeg, MB R3E 3P4, Canada

[4] Hlth Sci Ctr, 799 JBRC,715 McDermot Ave, Winnipeg, MB R3E 3P4, Canada

来源：

MICROMACHINES | 2022年 / 13卷 / 10期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

chemotaxis; T cells; migration; microfluidic device; multi-channel; NEUTROPHIL CHEMOTAXIS; SYSTEM;

D O I：

10.3390/mi13101567

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Immune surveillance is dependent on lymphocyte migration and targeted recruitment. This can involve different modes of cell motility ranging from random walk to highly directional environment-guided migration driven by chemotaxis. This study protocol describes a flow-based microfluidic device to perform quantitative multiplex cell migration assays with the potential to investigate in real time the migratory response of T cells at the population or single-cell level. The device also allows for subsequent in situ fixation and direct fluorescence analysis of the cells in the microchannel.

引用

页数：13

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