Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

被引：9

作者：

Salaimeh, Ahmad A. ^{[1
]}

Campion, Jeffrey J. ^{[2
,3
]}

Gharaibeh, Belal Y. ^{[1
]}

Evans, Martin E. ^{[2
,3
]}

Saito, Kozo ^{[1
]}

机构：

[1] Univ Kentucky, Inst Res Technol Dev IR4TD, Coll Engn, Lexington, KY 40536 USA

[2] Univ Kentucky, Coll Med, Div Infect Dis, Dept Internal Med, Lexington, KY USA

[3] Vet Affairs Med Ctr, Lexington, KY USA

来源：

INFRARED PHYSICS & TECHNOLOGY | 2012年 / 55卷 / 01期

关键词：

Infrared thermography; Energy content; Real-time; Bacterial quantification; Staphylococcus aureus; BACTERIA; MICROORGANISMS; BIOCONVECTION;

D O I：

10.1016/j.infrared.2011.11.004

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia colt, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to similar to 4 x 10(8) CFU/ml. The EC of S. aureus was similar to 2-fold higher than that of E. colt at comparable cell concentrations suggesting that IRT may be used to distinguish genera. Published by Elsevier B.V.

引用

页码：170 / 172

页数：3

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