Bridging the gap between PCR detection of Mycobacterium tuberculosis complex and tuberculosis diagnosis

被引：0

作者：

Duarte, E. L. ^{[2
]}

Paixao, E. ^{[3
]}

David, S. ^{[1
]}

机构：

[1] Inst Nacl Saude Dr Ricardo Jorge, Dept Genet, P-1649016 Lisbon, Portugal

[2] Univ Evora, Inst Ciencias Agr & Ambientais Mediterranicas, Escola Ciencias & Tecnol, Evora, Portugal

[3] Inst Nacl Saude Dr Ricardo Jorge, Dept Epidemiol, P-1649016 Lisbon, Portugal

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2012年 / 16卷 / 03期

关键词：

Mycobacterium tuberculosis complex; real-time PCR; acid-fast smear microscopy; diagnosis; tuberculosis; ACID AMPLIFICATION TESTS; PULMONARY TUBERCULOSIS; METAANALYSIS; MENINGITIS;

D O I：

10.5588/ijtld.11.0239

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The growing demand for rapid diagnosis of tuberculosis (TB) has led to the incorporation of nucleic acid amplification (NAA) tests in case definitions. The objective of this study was to evaluate the contribution of a real-time polymerase chain reaction (PCR) assay in providing a result predictive of a confirmed TB case. Respiratory and extra-pulmonary specimens (n = 308) were subjected to NAA, culture and smear microscopy. Qualitative PCR assessment, translated by an increase in NAA cycles, disregarding template copy number, resulted in an increase in confirmed cases, helping to bridge the gap between the test's analytical performance and its actual performance in TB diagnosis.

引用

页码：391 / 393

页数：3

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