Selective quantification of human DNA by real-time PCR of FOXP2

被引：9
作者：
Soejima, Mikiko [1 ]
Hiroshige, Kenichi [1 ,2 ]
Yoshimoto, Joji [1 ]
Koda, Yoshiro [1 ]
机构：
[1] Kurume Univ, Sch Med, Dept Forens Med & Human Genet, Kurume, Fukuoka 8300011, Japan
[2] Fukuoka Prefectural Police Headquarters, Forens Sci Lab, Hakata Ku, Fukuoka 8128576, Japan
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2012年 / 6卷 / 04期
基金：
日本学术振兴会;
关键词：
Real-time PCR; Human identification; Quantification of DNA; TaqMan; POLYMERASE-CHAIN-REACTION; DEVELOPMENTAL VALIDATION; EVOLUTION; NUCLEAR;
D O I：
10.1016/j.fsigen.2011.09.006
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We established a simple quantitative PCR procedure with high specificity and sensitivity using TaqMan probes targeting the FOXP2 sequence. This assay distinguished human and nonhuman, including primates, samples with the exception of mouse, turtle, lizard, and fishes. However, the specific amplification of mouse, lizard, and turtle fragments of FOXP2 could be confirmed by electrophoresis after real-time PCR. Because the C-T values obtained for human DNA were not affected by contaminating animal DNA at concentrations up to 30 times that of human DNA, we were able to estimate the concentration of human DNA in mixed specimens. This assay provides a reliable and useful method for routine quantification of human-specific DNA in forensic practice. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：447 / 451
页数：5
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