Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay

被引：100

作者：

Beattie, SH ^{[1
]}

Williams, AG ^{[1
]}

机构：

[1] Hannah Res Inst, Ayr KA6 5HL, Scotland

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 03期

关键词：

D O I：

10.1046/j.1365-2672.1999.00498.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An improved qualitative cell cytotoxicity assay for the detection of Bacillus cereus emetic and enterotoxin is described. The presence of toxin in culture supernatant fluids was detected by measurement with the tetrazolium salt MTT, as it adversely affects the metabolic status of cultured CHO cells. Psychrotrophic B. cereus isolates (65) were assessed for toxin production using the cytotoxicity assay, and 91% of culture supernatant fluids were cytotoxic. Toxin assessment using BCET-RPLA and ELISA immunoassays indicated that 51% and 85% of the cultures, respectively, were toxigenic. There were pronounced strain differences in the amount of toxin produced by the B. cereus isolates. Some isolates of B. circulans, B. laterosporus/cereus, B. lentus, B. licheniformis, B. mycoides, B. subtilis and B. thuringiensis were also toxigenic.

引用

页码：221 / 225

页数：5

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