A Cationic Contrast Agent in X-ray Imaging of Articular Cartilage: Pre-Clinical Evaluation of Diffusion and Attenuation Properties

被引：3

作者：

Fantoni, Simone ^{[1
]}

Gabucci, Ilenia ^{[1
]}

Cardarelli, Paolo ^{[2
]}

Paterno, Gianfranco ^{[2
,3
]}

Taibi, Angelo ^{[3
]}

Cristofori, Virginia ^{[4
]}

Trapella, Claudio ^{[4
]}

Bazzani, Armando ^{[1
]}

Assenza, Marta ^{[5
]}

Bonacorsi, Alice Zanna ^{[5
]}

Conti, Daniele ^{[5
]}

Baruffaldi, Fabio ^{[5
]}

机构：

[1] Univ Bologna, Dept Phys & Astron, Alma Mater Studiorum, I-40127 Bologna, Italy

[2] Ist Nazl Fis Nucl INFN, Div Ferrara, I-44122 Ferrara, Italy

[3] Univ Ferrara, Dept Phys & Earth Sci, I-44121 Ferrara, Italy

[4] Univ Ferrara, Dept Chem Pharmaceut & Agr Sci, I-44121 Ferrara, Italy

[5] IRCCS Ist Ortoped Rizzoli, Med Technol Lab, I-40136 Bologna, Italy

来源：

DIAGNOSTICS | 2022年 / 12卷 / 09期

关键词：

X-ray imaging; articular cartilage; contrast agent; contrast-enhanced computed tomography; proteoglycans; post-traumatic osteoarthritis; ENHANCED COMPUTED-TOMOGRAPHY; BIOMECHANICAL PROPERTIES; POSTTRAUMATIC OSTEOARTHRITIS; CT; GLYCOSAMINOGLYCANS; PROTEOGLYCANS; MORPHOLOGY; SECTIONS; DENSITY; CHARGE;

D O I：

10.3390/diagnostics12092111

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of this study was the preliminary assessment of a new cationic contrast agent, the CA4+, via the analysis of spatial distribution in cartilage of ex vivo bovine samples, at micrometer and millimeter scale. Osteochondral plugs (n = 18) extracted from bovine stifle joints (n = 2) were immersed in CA4+ solution up to 26 h. Planar images were acquired at different time points, using a microCT apparatus. The CA4+ distribution in cartilage and saturation time were evaluated. Tibial plates from bovine stifle joints (n = 3) were imaged with CT, before and after 24 h-CA4+ bath immersion, at different concentrations. Afterward, potential CA4+ washout from cartilage was investigated. From microCT acquisitions, the CA4+ distribution differentiated into three distinct layers inside the cartilage, reflecting the spatial distribution of proteoglycans. After 24 h of diffusion, the iodine concentration reached in cartilage was approximately seven times that of the CA4+ bath. The resulting saturation time was 1.9 +/- 0.9 h and 2.6 +/- 2.9 h for femoral and tibial samples, respectively. Analysis of clinical CT acquisitions confirmed overall contrast enhancement of cartilage after 24 h immersion, observed for each CA4+ concentration. Distinct contrast enhancement was reached in different cartilage regions, depending on tissue's local features. Incomplete but remarkable washout of cartilage was observed. CA4+ significantly improved cartilage visualization and its qualitative analysis.

引用

页数：17

共 54 条

[1] ENERGY-SELECTIVE RECONSTRUCTIONS IN X-RAY COMPUTERIZED TOMOGRAPHY [J].