Electrophysiological investigation of human embryonic stem cell derived neurospheres using a novel spike detection algorithm

被引：20

作者：

Mayer, Margot ^{[1
]}

Arrizabalaga, Onetsine ^{[2
]}

Lieb, Florian ^{[3
]}

Ciba, Manuel ^{[1
]}

Ritter, Sylvia ^{[2
]}

Thielemann, Christiane ^{[1
]}

机构：

[1] Univ Appl Sci Aschaffenburg, BioMEMS Lab, Wurzburger Str 45, D-63743 Aschaffenburg, Germany

[2] GSI Helmholtz Ctr Heavy Ion Res, Biophys Div, Planckstr 1, D-64291 Darmstadt, Germany

[3] Univ Appl Sci Aschaffenburg, Dept Technomath, Wurzburger Str 45, D-63743 Aschaffenburg, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 2018年 / 100卷

关键词：

Microelectrode array; Neurosphere; Spike detection algorithm; Human embryonic stem cell-derived neurons; SWTFEO; 3D culture; CORTICAL NETWORKS; IN-VITRO; PATTERNS; NEURONS; DIFFERENTIATION; EXPRESSION; PLATFORM; CULTURES; BURST;

D O I：

10.1016/j.bios.2017.09.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Microelectrode array (MEA) technology in combination with three-dimensional (3D) neuronal cell models derived from human embryonic stem cells (hESC) provide an excellent tool for neurotoxicity screening. Yet, there are significant challenges in terms of data processing and analysis, since neuronal signals have very small amplitudes and the 3D structure enhances the level of background noise. Thus, neuronal signal analysis requires the application of highly sophisticated algorithms. In this study, we present a new approach optimized for the detection of spikes recorded from 3D neurospheres (NS) with a very low signal-to-noise ratio. This was achieved by extending simple threshold-based spike detection utilizing a highly sensitive algorithm named SWTTEO. This analysis procedure was applied to data obtained from hESC-derived NS grown on MEA chips. Specifically, we examined changes in the activity pattern occurring within the first ten days of electrical activity. We further analyzed the response of NS to the GABA receptor antagonist bicuculline. With this new algorithm method we obtained more reliable results compared to the simple threshold-based spike detection.

引用

页码：462 / 468

页数：7

共 39 条

[11] Human embryonic stem cell-derived neuronal cells form spontaneously active neuronal networks in vitro [J].

Heikkila, Teemu J. ;

Yla-Outinen, Laura ;

Tanskanen, Jarno M. A. ;

Lappalainen, Riikka S. ;

Skottman, Heli ;

Suuronen, Rfitta ;

Mikkonen, Jarno E. ;

Hyttinen, Jari A. K. ;

Narkilahti, Susanna .

EXPERIMENTAL NEUROLOGY, 2009, 218 (01) :109-116

[12] Interactions of B16F10 melanoma cells aggregated on a cellulose substrate [J].

Hindie, M. ;

Vayssade, M. ;

Dufresne, M. ;

Queant, S. ;

Warocquier-Clerout, R. ;

Legeay, G. ;

Vigneron, P. ;

Olivier, V. ;

Duval, J. -L. ;

Nagel, M. -D. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 99 (01) :96-104

[13] Niche-dependent development of functional neuronal networks from embryonic stem cell-derived neural populations [J].

Illes, Sebastian ;

Theiss, Stephan ;

Hartung, Hans-Peter ;

Siebler, Mario ;

Dihne, Marcel .

BMC NEUROSCIENCE, 2009, 10

[14] Simultaneous induction of pathway-specific potentiation and depression in networks of cortical neurons [J].

Jimbo, Y ;

Tateno, T ;

Robinson, HPC .

BIOPHYSICAL JOURNAL, 1999, 76 (02) :670-678

[15] Microelectrode arrays: A physiologically based neurotoxicity testing platform for the 21st century [J].

Johnstone, Andrew F. M. ;

Gross, Guenter W. ;

Weiss, Dieter G. ;

Schroeder, Olaf H. -U. ;

Gramowski, Alexandra ;

Shafer, Timothy J. .

NEUROTOXICOLOGY, 2010, 31 (04) :331-350

[16] Triangular neuronal networks on microelectrode arrays: an approach to improve the properties of low-density networks for extracellular recording [J].

Jungblut, Melanie ;

Knoll, Wolfgang ;

Thielemann, Christiane ;

Pottek, Mark .

BIOMEDICAL MICRODEVICES, 2009, 11 (06) :1269-1278

[17] ASPIRIN-INDUCED TERATOGENESIS - A UNIQUE PATTERN OF CELL-DEATH AND SUBSEQUENT POLYDACTYLY IN THE RAT [J].

KLEIN, KL ;

SCOTT, WJ ;

WILSON, JG .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1981, 216 (01) :107-112

[18] Thalidomide metabolism and hydrolysis: Mechanisms and implications [J].

Leppert, Erin R. ;

Smith, Nicola F. ;

Cox, Michael C. ;

Scripture, Charity D. ;

Figg, William D. .

CURRENT DRUG METABOLISM, 2006, 7 (06) :677-685

[19]

Li X., 2009, P SPIE INT SOC OPT E, V7176

[20] A stationary wavelet transform and a time-frequency based spike detection algorithm for extracellular recorded data [J].

Lieb, Florian ;

Stark, Hans-Georg ;

Thielemann, Christiane .

JOURNAL OF NEURAL ENGINEERING, 2017, 14 (03)

← 1 2 3 4 →