Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae

被引:102
|
作者
Rigoulet, M
Aguilaniu, H
Avéret, N
Bunoust, O
Camougrand, N
Grandier-Vazeille, X
Larsson, C
Pahlman, IL
Manon, S
Gustafsson, L
机构
[1] Univ Victor Segalen, IBGC, CNRS, UMR 5095, F-33077 Bordeaux, France
[2] Univ Gothenburg, Lundberg Lab, Dept Cellular & Mol Biol Microbiol, Gothenburg, Sweden
[3] Chalmers Univ Technol, Lundberg Lab, Dept Mol Biotechnol, S-41296 Gothenburg, Sweden
关键词
yeast; in situ mitochondria; NADH dehydrogenases; respiratory chain; channeling and kinetic regulation; supramolecular complexes;
D O I
10.1023/B:MCBI.0000009888.79484.fd
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Keeping a cytosolic redox balance is a prerequisite for living cells in order to maintain a metabolic activity and enable growth. During growth of Saccharomyces cerevisiae, an excess of NADH is generated in the cytosol. Aerobically, it has been shown that the external NADH dehydrogenase, Nde1p and Nde2p, as well as the glycerol-3-phosphate dehydrogenase shuttle, comprising the cytoplasmic glycerol-3-phosphate dehydrogenase, Gpd1p, and the mitochondrial glycerol-3-phosphate dehydrogenase, Gut2p, are the most important mechanisms for mitochondrial oxidation of cytosolic NADH. In this review we summarize the recent results showing (i) the contribution of each of the mechanisms involved in mitochondrial oxidation of the cytosolic NADH, under different physiological situations; (ii) the kinetic and structural properties of these metabolic pathways in order to channel NADH from cytosolic dehydrogenases to the inner mitochondrial membrane and (iii) the organization in supramolecular complexes and, the peculiar ensuing kinetic regulation of some of the enzymes ( i. e. Gut2p inhibition by external NADH dehydrogenase activity) leading to a highly integrated functioning of enzymes having a similar physiological function. The cell physiological consequences of such an organized and regulated network are discussed.
引用
收藏
页码:73 / 81
页数:9
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