Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

被引：85
作者：
Shelbourne, Montserrat [1 ]
Chen, Xiong [1 ]
Brown, Tom [1 ]
El-Sagheer, Afaf H. [1 ,2 ]
机构：
[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England
[2] Suez Canal Univ, Chem Branch, Dept Sci & Math, Fac Petr & Min Engn, Suez 43721, Egypt
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 22期
关键词：
SIDE-CHAINS; CHEMISTRY; FUNCTIONALIZATION; HAIRPIN; GLYCANS; (TL)DNA; ANALOG;
D O I：
10.1039/c1cc10743g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
引用
收藏
页码：6257 / 6259
页数：3
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