Antioxidant capacity of 12 major soybean isoflavones and their bioavailability under simulated digestion and in human intestinal Caco-2 cells

被引:42
|
作者
Kim, Mi-Seon [1 ]
Jung, Young Sung [2 ]
Jang, Davin [1 ]
Cho, Chi Heung [3 ]
Lee, Sang-Hoon [3 ]
Han, Nam Soo [4 ]
Kim, Dae-Ok [1 ,2 ]
机构
[1] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 17104, South Korea
[2] Kyung Hee Univ, Dept Food Sci & Biotechnol, Yongin 17104, South Korea
[3] Korea Food Res Inst, Div Funct Food Res, Wonju 55365, South Korea
[4] Chungbuk Natl Univ, Brain Korea 21 Ctr Biohlth Ind, Dept Food Sci & Biotechnol, Chungbuk 28644, South Korea
关键词
Bioaccessibility; Cellular accumulation; Digestion stability; Isoflavone conjugate; Permeability; Transwell system; IN-VITRO DIGESTION; SOY ISOFLAVONES; ABSORPTION; FLAVONOIDS; TRANSPORT; FOOD; PERMEABILITY; METABOLISM; AGLYCONES; GENISTEIN;
D O I
10.1016/j.foodchem.2021.131493
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Soy isoflavones (SIs) show various health benefits, such as antioxidant and estrogenic effects. It is important to understand the bioaccessibility and bioavailability of SIs due to the close relation to their bioactivities. In this study, the antioxidant capacity, bioaccessibility, and bioavailability of 12 SIs were evaluated using radicalscavenging methods, simulations of human digestion, and Caco-2 cells in Transwell, respectively. All SIs were stable (91.1-99.2%) under gastric digestion conditions compared with the control (100%), whereas acetyl and malonyl conjugates were unstable (38.5% and 65.5%, respectively) under small intestinal digestion conditions. SI aglycones showed higher permeability (7-15 times) and cellular accumulation (8.8 times) than their glucosides. A small amount of SI conjugates was intact in the cell and in the basolateral side of each Transwell. These results suggest that SI conjugates, especially malonyl and acetyl forms, have incidental bioactivity after being metabolized to aglycones inside the cell.
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页数:10
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