Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells

被引：0

作者：

Choe, Mu Seog ^{[1
]}

Yeo, Han Cheol ^{[1
]}

Youm, Jae Boum ^{[2
]}

Choi, Seung Hak ^{[3
]}

Choi, Won-Young ^{[1
]}

Kim, So Jin ^{[1
]}

Oh, Seung Tack ^{[1
]}

Han, Ho Jae ^{[4
]}

Baek, Kyung Min ^{[5
]}

Kim, Joong Sun ^{[6
]}

Lim, Kyung Seob ^{[7
]}

Chang, Woochul ^{[8
]}

Lee, Min Young ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea

[2] Inje Univ, Coll Med, Cardiovasc & Metab Dis Ctr, Dept Physiol & Biophys, Busan 47392, South Korea

[3] Inje Univ, Coll Med, Dept Physiol, Busan 47392, South Korea

[4] Seoul Natl Univ, Coll Vet Med, Dept Vet Physiol, Seoul 08826, South Korea

[5] Daegu Haany Univ, Coll Oriental Med, Dept Cardiovasc & Neurol Dis, Daegu 42158, South Korea

[6] Korea Inst Oriental Med, K Herb Res Ctr, Daejeon 34054, South Korea

[7] Korea Res Inst Biosci & Biotechnol, Futurist Anim Resource & Res Ctr, Cheongju 28116, South Korea

[8] Pusan Natl Univ, Coll Educ, Dept Biol Educ, Busan 46241, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2021年 / 26卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

cardiac differentiation; human embryonic stem cells; cyclosporin A; Wnt; beta-catenin signaling; SMALL-MOLECULE; PROLIFERATION; CARDIOMYOCYTES; PATHWAY;

D O I：

10.1007/s12257-020-0296-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Efficient cardiac differentiation of human pluripotent stem cells (hPSCs) is essential for their use in the field of basic research as well as in cell-based therapy. Regulation of the Wnt/beta-catenin pathway is a key step in the process of cardiac differentiation of hPSCs. In this study, we reported cyclosporin A (CsA), a strong immunosuppressor, as an inducer of cardiac differentiation in human embryonic stem cells (hESCs). Our results showed that CsA promoted cardiac differentiation in the mesodermal differentiation stage of hESCs and that this effect was mediated by the inhibition of Wnt/beta-catenin signaling. Therefore, our results provide novel information about CsA as a Wnt/beta-catenin inhibitor as well as a strategy for the cardiac differentiation of hESCs.

引用

页码：786 / 794

页数：9