Fluorescence resonance energy transfer (FRET): theory and experiments

被引:41
作者
Chirio-Lebrun, MC
Prats, M
机构
[1] CNRS, IPBS, F-31077 Toulouse, France
[2] Univ Toulouse 3, F-31077 Toulouse, France
来源
BIOCHEMICAL EDUCATION | 1998年 / 26卷 / 04期
关键词
D O I
10.1016/S0307-4412(98)80010-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence resonance energy transfer (FRET) is a powerful biophysical technique permitting macromolecular interactions between fluorescent molecules in close contact with each other to be analysed. Studies of the kinetics of association/dissociation between biological macromolecules may he carried out using this technique. Distances of interaction between donor-acceptor may be estimated by the FRET approach. (C) 1998 IUBMB. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:320 / 323
页数:4
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