FCS 3.1 Implementation Guidance

被引:5
作者
Bray, Chris [2 ]
Spidlen, Josef [1 ]
Brinkman, Ryan R. [1 ]
机构
[1] British Columbia Canc Res Ctr, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada
[2] Ver Software House, Topsham, ME USA
关键词
flow cytometry; FCS; data standard; file format; bioinformatics; DATA FILE STANDARD; FLOW-CYTOMETRY;
D O I
10.1002/cyto.a.22018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The Flow Cytometry Standard (FCS) format was developed back in 1984. Since then, FCS became the standard file format supported by all flow cytometry software and hardware vendors. Over the years, updates were incorporated to adapt to technological advancements in both flow cytometry and computing technologies. However, flexibility in how data may be stored in FCS has led to implementation difficulties for instrument vendors and third party software developers. In this technical note, we are providing implementation guidance and examples related to FCS 3.1, the latest version of the standard. By publishing this text, we intend to prevent potential compatibility issues that could be faced when implementing the FCS spillover and preferred display keywords that have arisen during discussions among some implementers. (c) 2012 International Society for Advancement of Cytometry.
引用
收藏
页码:523 / 526
页数:4
相关论文
共 8 条
[1]   HyperLog - A flexible log-like transform for negative, zero, and positive valued data [J].
Bagwell, CB .
CYTOMETRY PART A, 2005, 64A (01) :34-42
[2]  
DEAN PN, 1990, CYTOMETRY, V11, P323
[3]   A PROPOSAL FOR A FLOW CYTOMETRIC DATA FILE STANDARD [J].
MURPHY, RF ;
CHUSED, TM .
CYTOMETRY, 1984, 5 (05) :553-555
[4]   A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data [J].
Parks, David R. ;
Roederer, Mario ;
Moore, Wayne A. .
CYTOMETRY PART A, 2006, 69A (06) :541-551
[5]  
Seamer LC, 1997, CYTOMETRY, V28, P118, DOI 10.1002/(SICI)1097-0320(19970601)28:2<118::AID-CYTO3>3.0.CO
[6]  
2-B
[7]   Data File Standard for Flow Cytometry, Version FCS 3.1 [J].
Spidlen, Josef ;
Moore, Wayne ;
Parks, David ;
Goldberg, Michael ;
Bray, Chris ;
Bierre, Pierre ;
Gorombey, Peter ;
Hyun, Bill ;
Hubbard, Mark ;
Lange, Simon ;
Lefebvre, Ray ;
Leif, Robert ;
Novo, David ;
Ostruszka, Leo ;
Treister, Adam ;
Wood, James ;
Murphy, Robert E. ;
Roederer, Mario ;
Sudar, Damir ;
Zigon, Robert ;
Brinkman, Ryan R. .
CYTOMETRY PART A, 2010, 77A (01) :97-100
[8]   Gating-ML: XML-Based Gating Descriptions in Flow Cytometry [J].
Spidlen, Josef ;
Leif, Robert C. ;
Moore, Wayne ;
Roederer, Mario ;
Brinkman, Ryan R. .
CYTOMETRY PART A, 2008, 73A (12) :1151-1157