The application of microarray technology to the identification of Tc1-like element sequences in fish genomes

被引:4
作者
Wenne, Roman [1 ]
Handschuh, Luiza [2 ,3 ]
Pocwierz-Kotus, Anita [1 ]
Urbaniak, Radoslaw [4 ]
Formanowicz, Piotr [2 ,4 ]
Calkiewicz, Joanna [1 ]
Brzozowska, Katarzyna [5 ]
Figlerowicz, Marek [2 ]
Wegrzyn, Grzegorz [5 ]
Wrobel, Borys [1 ,6 ]
机构
[1] Polish Acad Sci, Inst Oceanol, Dept Genet & Marine Biotechnol, PL-81712 Sopot, Poland
[2] Polish Acad Sci, Inst Bioorgan Chem, Poznan, Poland
[3] Poznan Univ Med Sci, Poznan, Poland
[4] Poznan Univ Tech, Inst Comp Sci, Poznan, Poland
[5] Univ Gdansk, Fac Biol, Dept Mol Biol, PL-80952 Gdansk, Poland
[6] Adam Mickiewicz Univ, Lab Bioinformat, Inst Mol Biol & Biotechnol, Poznan, Poland
关键词
DNA microarray; fish; genome; Tc1; transposon; TRANSPOSABLE ELEMENTS; ZEBRAFISH; FAMILY; EVOLUTION; MOBILE;
D O I
10.1080/17451000.2010.527990
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The phylogenetic relationships between Tc1 transposable elements have previously been reported for the genomes of some fish species. However, research in this field has been hindered by the low number of fish genome sequences available in databases. The application of a DNA microarray as a universal tool for Tc1 transposon sequence analysis in fish genomes is described here. A prototype oligonucleotide microarray was constructed and used to compare samples of genomic DNA isolated from selected fish species. These results, combined with earlier reported molecular analysis of Tc1 showed the usefulness of DNA microarray in the screening of transposon sequences.
引用
收藏
页码:466 / 477
页数:12
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