Large-scale extraction of rhamnogalacturonan I from industrial potato waste

被引：40

作者：

Byg, Inge ^{[1
]}

Diaz, Jerome ^{[1
]}

Ogendal, Lars Holm ^{[2
]}

Harholt, Jesper ^{[1
]}

Jorgensen, Bodil ^{[1
]}

Rolin, Claus

Svava, Rikke ^{[1
,3
]}

Ulvskov, Peter ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Plant Biol & Biotechnol, LIFE, DK-1871 Frederiksberg C, Denmark

[2] Univ Copenhagen, Dept Basic Sci & Environm, LIFE, DK-1871 Frederiksberg C, Denmark

[3] Univ Copenhagen, Dept Cellular & Mol Med, HEALTH, DK-2200 Copenhagen, Denmark

来源：

FOOD CHEMISTRY | 2012年 / 131卷 / 04期

关键词：

Rhamnogalacturonan I; Potato; Polygalacturonase; Pectin; Enzymatic extraction; SOYBEAN SOLUBLE POLYSACCHARIDES; HAIRY RAMIFIED REGIONS; ENZYMATIC DEGRADATION; CELL-WALLS; ACIDIC CONDITIONS; PECTINS; GALACTOSIDASE; ACETYLATION; MUCILAGE; APPLE;

D O I：

10.1016/j.foodchem.2011.09.106

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Potato pulp is rich in dietary fibres and is an underutilised material produced in large quantities by the potato starch factories. Potato fibres are especially rich in rhamnogalacturonan I (RG I). RC I is a pectic polysaccharide with a high degree of branching and until now undegraded RG I has only been extracted in small amounts limiting the application possibilities for RG I. The present paper describes a large-scale extraction process providing large quantities of undegraded RG I readily available. The extraction process includes enzymatic starch removal using purified Termamyl, enzymatic RG I solubilisation using a highly purified polygalacturonase, and finally purification using depth filtration and ultrafiltration. The extracted RG I has a high molecular weight and a monosaccharide composition comparable to RG I extracted by analytical extraction procedures. The large amount of RG I available by the presented method allows for thorough structure-function analyses and tailoring of RG I to specific functionalities. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1207 / 1216

页数：10

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