Detailed Structural Characterization of the Lipooligosaccharide from the Extracellular Membrane Vesicles of Shewanella vesiculosa HM13

被引：10

作者：

Di Guida, Rossella ^{[1
]}

Casillo, Angela ^{[1
,2
]}

Yokoyama, Fumiaki ^{[3
]}

Kawamoto, Jun ^{[3
]}

Kurihara, Tatsuo ^{[3
]}

Corsaro, Maria Michela ^{[1
,2
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia 4, I-80126 Naples, Italy

[2] Univ Naples Federico II, Task Force Blue Italian Growth BigFedII, I-80126 Naples, Italy

[3] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

来源：

MARINE DRUGS | 2020年 / 18卷 / 05期

关键词：

Shewanella vesiculosa; cold-adapted bacterium; extracellular membrane vesicles; mass spectrometry; lipid A; core oligosaccharide; LIPOPOLYSACCHARIDES; BIOGENESIS; PROTEINS;

D O I：

10.3390/md18050231

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Bacterial extracellular membrane vesicles (EMVs) are membrane-bound particles released during cell growth by a variety of microorganisms, among which are cold-adapted bacteria. Shewanella vesiculosa HM13, a cold-adapted Gram-negative bacterium isolated from the intestine of a horse mackerel, is able to produce a large amount of EMVs. S. vesiculosa HM13 has been found to include a cargo protein, P49, in the EMVs, but the entire mechanism in which P49 is preferentially included in the vesicles has still not been completely deciphered. Given these premises, and since the structural study of the components of the EMVs is crucial for deciphering the P49 transport mechanism, in this study the complete characterization of the lipooligosaccharide (LOS) isolated from the cells and from the EMVs of S. vesiculosa HM13 grown at 18 degrees C is reported. Both lipid A and core oligosaccharide have been characterized by chemical and spectroscopic methods.

引用

页数：11

共 26 条

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