New variants of E6 and E7 oncogenes of human papillomavirus type 31 identified in Northeastern Brazil

被引：20

作者：

Chagas, Barbara S. ^{[1
]}

Batista, Marcus V. A. ^{[1
]}

Guimaraes, Vilma ^{[2
]}

Balbino, Valdir Q. ^{[1
]}

Crovella, Sergio ^{[1
,3
,4
]}

Freitas, Antonio C. ^{[1
,3
]}

机构：

[1] Univ Fed Pernambuco, Dept Genet, Recife, PE, Brazil

[2] Inst Integral Med Prof Fernando Figueira, Recife, PE, Brazil

[3] Univ Fed Pernambuco, Lab Immunopathol Keizo Azami, Recife, PE, Brazil

[4] Genet Serv IRCCS Burlo Garofolo, Trieste, Italy

来源：

GYNECOLOGIC ONCOLOGY | 2011年 / 123卷 / 02期

关键词：

Human papillomavirus variants; Genetic diversity; Cervical cancer; CERVICAL INTRAEPITHELIAL NEOPLASIA; HUMAN-PAPILLOMAVIRUS-16; E6; SEQUENCE VARIANTS; BINDING-SITES; RISK; PREDICTION; LESIONS; CANCER; GENE; POLYMORPHISM;

D O I：

10.1016/j.ygyno.2011.07.008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objective. We sought to characterize E6 and E7 oncogenes genetic variability of HPV-31 isolated from cervical scraping samples of Northeastern Brazilian women. Methods. E6 and E7 were amplified with specific primers, cloned and sequenced. The sequences obtained were aligned with the GenBank reference sequences with the aim of evaluating the possible genetic variants. Results. We identified several genetic variants in E6 and E7 sequences from HPV-31 positive women. Three nucleotide changes in E6 were described for the first time in this study. Some nucleotide changes were non-synonymous substitutions. Conclusion. The knowledge of region/country HPV specific genetic variations is relevant to understand the epidemiology and the development of effective vaccines. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：284 / 288

页数：5

共 32 条

[11] Classification of papillomaviruses [J].

de Villiers, EM ;

Fauquet, C ;

Broker, TR ;

Bernard, HU ;

zur Hausen, H .

VIROLOGY, 2004, 324 (01) :17-27

[12]

Hu XR, 2001, J PATHOL, V195, P307, DOI 10.1002/path.944

[13] Variants of the long control region and the E6 oncogene in European human papillomavirus type 16 isolates:: implications for cervical disease [J].

Kämmer, C ;

Tommasino, M ;

Syrjänen, S ;

Delius, H ;

Hebling, U ;

Warthorst, U ;

Pfister, H ;

Zehbe, I .

BRITISH JOURNAL OF CANCER, 2002, 86 (02) :269-273

[14]

KAST WM, 1994, J IMMUNOL, V152, P3904

[15] Human papillomavirus 16 E6, L1, L2 and E2 gene variants in cervical lesion progression [J].

Lee, K. ;

Magalhaes, I. ;

Clavel, C. ;

Briolat, J. ;

Birembaut, P. ;

Tommasino, M. ;

Zehbe, I. .

VIRUS RESEARCH, 2008, 131 (01) :106-110

[16]

Londesborough P, 1996, INT J CANCER, V69, P364

[17] The use of human papillomavirus typing in detection of cervical neoplasia in Recife (Brazil) [J].

Lorenzato, F ;

Ho, L ;

Terry, G ;

Singer, A ;

Santos, LC ;

Batista, RD ;

Lubambo, T .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2000, 10 (02) :143-150

[18] Enhanced oncogenicity of human papillomavirus type 16 (HPV16) variants in Japanese population [J].

Matsumoto, K ;

Yoshikawa, H ;

Nakagawa, S ;

Tang, XH ;

Yasugi, T ;

Kawana, K ;

Sekiya, S ;

Hirai, Y ;

Kukimoto, T ;

Kanda, T ;

Taketani, Y .

CANCER LETTERS, 2000, 156 (02) :159-165

[19] jModelTest: Phylogenetic model averaging [J].

Posada, David .

MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (07) :1253-1256

[20] Genetic Variability and Phylogeny of the High-Risk HPV-31,-33,-35,-52, and-58 in Central Brazil [J].

Raiol, Taina ;

Wyant, Patricia Soares ;

Santos De Amorim, Regina Maria ;

Cerqueira, Daniela Marreco ;

Milanezi, Natalia von Gal ;

Brigido, Marcelo de Macedo ;

Sichero, Laura ;

Fernandes Martins, Claudia Renata .

JOURNAL OF MEDICAL VIROLOGY, 2009, 81 (04) :685-692

← 1 2 3 4 →