Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

被引：0
|
作者：
Gavel, Olga Yu. [1 ]
Kladova, Anna V. [1 ]
Bursakov, Sergey A. [1 ]
Dias, Joao M. [1 ]
Texeira, Susana [1 ]
Shnyrov, Valery L. [1 ]
Moura, Jose J. G. [1 ]
Moura, Isabel [1 ]
Romao, Maria J. [1 ]
Trincao, Jose [1 ]
机构：
[1] Univ Nova Lisboa, Fac Ciencias & Tecnol, Centro Quim Fina & Biotechnol, Dept Quim,REQUIMTE, P-2829516 Caparica, Portugal
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷
关键词：
D O I：
10.1107/S1744309108008816
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Native zinc/cobalt-containing ATP sulfurylase ( ATPS; EC 2.7.7.4; MgATP: sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 angstrom resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.
引用
收藏
页码：593 / 595
页数：3
相关论文
共 50 条
  • [21] Crystallization and preliminary X-ray diffraction analysis of the 16-haem cytochrome of Desulfovibrio gigas
    Santos-Silva, T
    Diasa, JM
    Bourenkov, G
    Bartunik, H
    Moura, I
    Romao, MJ
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 968 - 970
  • [22] Purification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase
    Tsekoa, TL
    Sayed, MF
    Cameron, RA
    Sewell, BT
    Cowan, DA
    SOUTH AFRICAN JOURNAL OF SCIENCE, 2004, 100 (9-10) : 488 - 490
  • [23] Purification, crystallization and preliminary X-ray diffraction analysis of human phosphoserine phosphatase
    Peeraer, Y
    Rabijns, A
    Verboven, C
    Collet, JF
    Van Schaftingen, E
    De Ranter, C
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 133 - 134
  • [24] Purification, crystallization and preliminary X-ray analysis of the dissimilatory sulfite reductase from Desulfovibrio vulgaris Miyazaki F
    Ogata, Hideaki
    Shomura, Yasuhito
    Agrawal, Aruna Goenka
    Kaur, Amrit Pal
    Gaertner, Wolfgang
    Higuchi, Yoshiki
    Lubitz, Wolfgang
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 1470 - 1472
  • [25] The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum
    Dobson, Renwick C. J.
    Atkinson, Sarah C.
    Gorman, Michael A.
    Newman, Janet M.
    Parker, Michael W.
    Perugini, Matthew A.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 206 - 208
  • [26] Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii
    Inagaki, E
    Sakamoto, K
    Obayashi, N
    Terada, T
    Shirouzu, M
    Bessho, Y
    Kuroishi, C
    Kuramitsu, S
    Shinkai, A
    Yokoyama, S
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 169 - 171
  • [27] Purification, crystallization and preliminary X-ray diffraction analysis of the glyoxalase II from Leishmania infantum
    Trincao, Jose
    Silva, Marta Sousa
    Barata, Lidia
    Bonifacio, Cecilia
    Carvalho, Sandra
    Tomas, Ana Maria
    Ferreira, Antonio E. N.
    Cordeiro, Carlos
    Freire, Ana Ponces
    Romao, Maria Joao
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 805 - 807
  • [28] Overproduction, crystallization and preliminary X-ray diffraction analysis of probable ATP sulfurylase from Thermus thermophilus HB8
    Taguchi, Y
    Hoseki, J
    Kakuta, Y
    Fukuyama, K
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2003, 59 : 1645 - 1647
  • [29] Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45γ
    Zhang, Wenzheng
    Zhao, Mingzhuo
    Li, Jianhui
    Li, Xuemei
    Zeng, ZongHao
    Rao, Zihe
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 1070 - 1073
  • [30] Purification, crystallization and preliminary X-ray diffraction analysis of an archaeal ABC-ATPase
    Verdon, G
    Albers, SV
    Dijkstra, BW
    Driessen, AJM
    Thunnissen, AMWH
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 362 - 365
12345