Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

被引：6

作者：

Dahani, Mohamed ^{[1
]}

Barret, Laurie-Anne ^{[1
,2
]}

Raynal, Simon ^{[1
]}

Jungas, Colette ^{[2
]}

Pernot, Petra ^{[3
]}

Polidori, Ange ^{[1
]}

Bonnete, Francoise ^{[1
]}

机构：

[1] Avignon Univ, UMR 5247, CBSA, Inst Biomol Max Mousseron, F-84000 Avignon, France

[2] Lab Bioenerget Cellulaire Biol Vegetale & Microbi, UMR 7265, F-13108 St Paul Les Durance, France

[3] European Synchrotron Radiat Facil, F-38000 Grenoble, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷

关键词：

membrane protein; surfactant micelle; scattering techniques; phase diagram; crystallization; 2ND VIRIAL-COEFFICIENT; HEMIFLUORINATED SURFACTANTS; OMPF PORIN; FACIAL AMPHIPHILES; AQUEOUS-SOLUTIONS; DETERGENT; SOLUBILIZATION; STABILIZATION; SAXS; MACROMOLECULES;

D O I：

10.1107/S2053230X15009516

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-beta-M and F4H5-beta-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-beta-maltoside (DD-beta-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar inter-actions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

引用

页码：838 / 846

页数：9

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